摘要
以KRL-1(库尔勒香梨分离物)总RNA为模板,采用RT-PCR技术,扩增外壳蛋白(CP)基因的cDNA片段,将其克隆到pMD18-T载体上。通过序列测定,分析结果表明:KRL-1CP基因由714个核苷酸组成,编码237个氨基酸。KRL-1与ASGV其它分离物CP基因的核苷酸同源性为90%-93%,氨基酸同源性为94%-98%。根据KRL-1与其他分离物CP基因序列和生物学症状上的差异,可推断KRL-1存在较大的分子变异。
Kuala pear virus isolate （KRL-1） which was originally obtained from Kuala pear＇s petal by DU Yejuan was identified as Apple stem grooving virus （ASGV） by host range, ELISA, electron microscopy, and RT-PCR. However, KRL-1 was obviously different from other ASGV isolates in biological symptoms, so the coat protein gene （CP） of KRL-1 was cloned and sequenced. The sequence analysis showed that the capsid protein gene of KRL-1 contained 714 nucleotides, encoding a protein of 237 amino acids. The nucleotide and amino acid sequence of CP gene of this isolate shared 90% to 93% and 94% to 98% similarities to that of other ASGV isolates, respectively. The divergence of CP gene sequences and biological symptoms indicated that there was much molecular variability on KRL-1.
出处
《果树学报》
CAS
CSCD
北大核心
2005年第5期576-578,共3页
Journal of Fruit Science
基金
教育部优秀教师资助计划和全国优秀博士学位论文作者专项基金(200253)。
