摘要
采用瞬间表达技术分析了TaTBL、TaPK1和TaTST等3个抗病相关基因的功能。首先将这3个小麦抗病相关基因构建入高效表达载体,然后使用基因枪将目标基因和GUS基因载体同时导入到感白粉病小麦品种离体叶片表皮细胞中,用GUS基因标记阳性转化细胞。转化后接种白粉菌孢子,48h后观察转化阳性表皮细胞,研究抗病相关基因表达对白粉菌入侵及吸器形成产生的影响。结果表明,这3个基因在感病小麦品种叶片表皮细胞中的瞬间表达,对白粉菌侵入和吸器形成均有部分抑制作用,在一定程度上增强了表达细胞对白粉菌的抗性。
Transient expression system was used to analyze the functions of three resistance- related genes： TaTBL, TaPK1 and TaTST. Target genes were constructed into plant expression vectors and transformed into leaf epidermal cells of a powdery mildew-susceptible wheat variety by gene gun. GUS gene was co-transformed with target gene to mark the transformed cells. After transformation, leaf surface was inoculated with powdery mildew conidiospores. Forty eight hours after inoculation, penetration of the fungus and formation of haustoria in transformed cells were observed to evaluate the effects of the target gene＇ s products on the invasion of powdery mildew. The results implied that all these three genes,when transiently expressed in leaf epidermal cells of susceptible wheat variety,could partly inhibit the penetration of conidiospores and formation of haustoria,and to some extent increase the resistance of cells to powdery mildew.
基金
国家高技术研究发展计划(863)项目(编号:2001AA222152
2004AA222140)资助~~
关键词
小麦
白粉病
瞬间表达
抗病相关基因
wheat
powdery mildew
transient expression
resistance-related genes
