摘要
目的探讨贲门癌组织显微自体荧光特征及自体荧光在同体正常胃组织各层的分布和差异。方法采用激光扫描共聚焦显微镜以氩离子(Ar+)激光(Ex=488nm)和氦氖激光(He-Ne)(Ex=543nm)为激发光的双通道法对16例贲门癌手术标本(贲门癌组织与同体胃体组织)进行自体荧光图像分析。结果同体正常胃组织胃壁各层中,以黏膜下层荧光信号最强。固有层呈现较强的荧光信号,主要分布于腺体上。癌组织自体荧光信号与正常组织各层相比均显著减弱(P<0.01)。结论贲门癌组织的显微自体荧光与同体正常胃组织在形态、颜色、分布及荧光强度上都存在明显差异。
Objective To investigate the microscopic autofluorescent characteristics of cardiac cancer and autofluorescence distribution in different layers of gastric tissues. Methods A double-channel laser scanning confocal microscopy with Argon ion laser （excitation wavelength 488 nm） and Helium-Neon laser （excitation wavelength 543 nm） were used to detect the autofluorescence emitted from 16 surgical specimens of cardiac cancer and corresponding normal gastric tissue. The autofluorescence image was analyzed between the cardiac cancer tissue and normal gastric tissue. Results Autofluorescence was detected successfully in cardiac carcinoma and corresponding normal gastric corpus tissues of all 16 cases. In different layers of gastric tissue, fluorescence presented the strongest signal in submucosa, the second strong in laminal propria with fluorescence mostly distributed in the glands, fluorescence signal from gastric cancer was signiHcantly decreased compared with those in the different layers of normal tissues （ P 〈 0. 01 ）. Conclusion There are significant differences in the shape, color, distribution and fluorescence intensity of microscopic autofluorescence between cardiac cancer tissues and normal gastric corpus tissues.
出处
《中华胃肠外科杂志》
CAS
2005年第5期432-435,共4页
Chinese Journal of Gastrointestinal Surgery
基金
广东省医学科学技术研究基金资助项目(A2004418)
