摘要
用乙磷铝(fosetyl-Al)和紫外线(UV)照射处理葡萄叶片,通过测定处理组织中白藜芦醇(resveratrol)的含量调查葡萄中芪类化合物的诱导水平,以评价葡萄的抗毒素诱导反应。试验表明,离体的葡萄叶片用乙磷铝和UV处理后,白藜芦醇的含量明显提高,最大白藜芦醇诱导量出现在UV照射后的48小时;而单独使用乙磷铝对葡萄叶片的白藜芦醇的诱导没有明显反应,并且乙磷铝喷洒和UV照射两处理间没有表现出附加效应。乙磷铝处理田间生长的叶片,结果与离体叶片处理不同,白藜芦醇的含量大大的增加。进一步用乙磷铝和UV照射处理田间叶片,也表明乙磷铝对白藜芦醇有明显的诱导作用。因此, 我们认为,乙磷铝对白藜芦醇的诱导性对离体组织没有影响,只发生在正常生长的组织中。
Grapevine leaves were treated with fosetyl-Al and UV radiation to investigate the induction of stilbenic compounds so that the phytoalexin response was confirmed, detected by resveratrol production. Detached leaves, sprayed with different concentrations of fosetyl-Al and then dosed with UV radiation showed a positive resveratrol response, with a peak concentration developing at 48 hr after UV radiation, However, without the UV-elicitation event, fosetyl-Al had no effect on resveratrol, The effects of fosetyl-Al and UV radiation on resveratrol did not appear additive. Leaves were treated with fosetyl- Al on the vines, unlike detached ones, showed a direct effect on resveratrol production. Our data indicated that resveratrol production, induced by using fosetyl-Al, differed when leaves were treated in situ or in vitro; fosetyl-Al only induced resveratrol production when the leaf tissues were attached to the plant.
出处
《中外葡萄与葡萄酒》
2005年第4期12-16,共5页
Sino-Overseas Grapevine & Wine
基金
本研究受中国国际教育交流协会山东分会资助在新西兰林肯大学葡萄栽培与酒类研究中心完成。
