摘要
研究病史资料完整的２６例淋巴瘤患者Ｐ－糖蛋白表达与临床化疗效果的关系。用免疫组织化学ＡＢＣ法检测８４例淋巴瘤初诊患者活检标本中的Ｐ－糖蛋白表达水平。结果：８４例淋巴瘤组织中有１０例Ｐ－糖蛋白表达阳性，阳性率为１１．９％；Ｐ－糖蛋白阳性着色部位主要在细胞浆和细胞膜外侧；阳性细胞多呈片状分布，在阳性和阴性标本中，可见散在分布的阳性细胞。１９例霍奇金病（ＨＤ）中６例呈阳性，６５例非霍奇金淋巴瘤（ＮＨＬ）中Ｐ－糖蛋白阳性有４例。２６例病史资料完整的患者中４例Ｐ－糖蛋白阳性的ＨＤ患者化疗效果较差，３例ＰＲ，１例ＰＤ；４例Ｐ－糖蛋白阴性的ＨＤ患者疗效较好，２例ＣＲ，１例ＰＲ，１例ＮＣ．１８例Ｐ－糖蛋白阴性的ＮＨＬ患者中，疗效为８例ＣＲ，５例ＰＲ，２例ＮＣ，３例ＰＤ，有效率高达７２％。结论：Ｐ－糖蛋白表达阴性的淋巴瘤患者化疗效果较好。
PURPOSE In order to analyze the relationship between P-gp expression and the effect of chemotherapy.METHODS An immunohistochemical method was used to test P-glycoprotein(P-gp)expression in 84 cases of lymphoma.RESULTS The percentage of positive P-gp expression was 11.9% in 84 cases.Polyclonal antibody mdr(Ab-1) was stained positively in cytoplasm, but monoclonal antibody MRK-16 was immunostained in extracellular P-gp epitope. Most of positive cells was gathered in tumor areas, and some were scattered. P-gp expression was observed significantly more often in Hodgkin's disease (HD) (6/19) than in non-Hodgkin's lymphoma(NHL) (4/65). In patients suffered from HD, the chemotheraputic results showed that 3 cases were partial remission (PR),1 case was progressive disease (PD) in P-gp positivespecimens. However,2 cases were complete remission(CR),1 case was PR and 1 case was no change (NC) in another 4 P-gp negative specimens. In 18 NHL patients of P-gp negative samples,8 cases were CR, 5 cases were PR,2 cases were NC,3 cases were PD,effective rate (CR+PR)was 72%.CONCLUSIONS There appears to be an correlation of P-gp expression with the effect of chemotherapy. The differences of percentage of positive cells and degree of positive staining in positive cases indicate that there is heterogenicity in P-gp expression.
出处
《上海医科大学学报》
CSCD
1996年第1期46-48,共3页
Journal of Fudan University(Medical Science)
基金
校青年科学研究基金
关键词
淋巴瘤
免疫组化
P糖蛋白
lymphoma
immunohistochemistry
P-glycoprotein
chemotherapy
