摘要
研究ＳＲＳ鼠白血病／淋巴瘤克隆株（包括ＳＲＳ─８２亲系和ＳＡＣ─ⅡＢ２、ＳＡＣ─ⅡＣ３克隆株）癌基因和癌基因蛋白的表达，筛选此克隆株的癌基因表达谱型，为ＳＲＳ淋巴瘤株的实验性反义核酸治疗提供目标。用ＡＢＣ免疫组织化学方法筛选ＳＲＳ─８２亲系和ＳＡＣ─ⅡＢ２、ＳＡＣ─ⅡＣ３克隆株癌基因表达诺型。在ＳＲＳ克隆株中呈强表达的有ｃ─ｆｏｓ和ｃ─ｍｙｃ。ｃ─ｊｕｎ、ｒａｓ─ｐ２１和ｃ─ｅｒｂＢ─２呈中度表达，Ｐ５３和ｂｃｌ─２则不表达。同时用ＣＤ４和ＣＤ８细胞表面标记进研究，发现ＳＲＳ克隆株属于原始干细胞。结果提示：癌基因谱型的建立对ＳＲＳ淋巴瘤克隆株的实验性反义核酸治疗具有相当重要的作用，ｃ─ｆｏｓ和ｃ─ｍｙｃ可能是反义核酸治疗最好的癌基因靶。
PURPOSE The studies of oncogenes expression in a SRS─82 mouse Lymphoma cell line and SAC─ⅡB2,SAC─ⅡC3 clones have been less reported.We must establish a oncogene spectrum for finishing the experimental antisense treatment to SRS lymphoma.METHODS:SRS─82 mouse lymphoma cell line and SAC─ⅡB2,SAC─ⅡC3 clones were obtained from the Department of Pathophysiology,Shanghai Medical University.ABC immunohistochemical method was used.RESULTS Strong staining was found for c─fos and c─myc,medium staining for c─jun,ras─p21 and c─erbB─2,and negative reactions for P53 and bcl─2 in SRS─82 cell line and its clones,Cell surface marks(CD4 and CD8) of these two clones and their parent cell line were negative,all of them belong to primary stem cell origin.CONCLUSIONS Establishments of oncogene spectrum play an important role in the experimental antisense treatment in SRS lymphoma,and c─fos and c─myc were the best targets for the antisense treatment.
出处
《上海医科大学学报》
CSCD
1996年第1期7-9,共3页
Journal of Fudan University(Medical Science)
基金
国家教育委员会博士点基金
关键词
克隆细胞
癌基因
免疫组化
淋巴瘤
白血病
clonal cell
oncogene
antisense
immunohistochemistry
SRS lymphoma
