摘要
目的为使医院感染流行病学调查方法由生物学提高到分子生物学水平。方法选用不同限制性核酸内切酶(BamHI、HindⅢ、EeoRI),对绿脓假单胞菌主要生物型进行染色体 DNA 酶切,凝胶电泳后获得限制性核酸内切酶(REA)图谱。应用聚合酶链反应(PCR)技术从 E.Coli 中分别扩增出16s、23s 核糖体核糖核酸(rDNA)基因,以[α-P^(32)]dATP 经缺口平移法标记 rDNA 作为广谱探针,经 Southern 杂交,获得 rDNA 指纹图谱。结果在同一医院中,不同病区患者分离菌和环境分离菌的REA 和 rDNA 图谱不同,而同一病区患者分离菌往往相同,并与环境污染有关。结论该方法对医院感染病原菌分型、精确确定传染源、阻断传播途径、控制和预防医院感染具有重要指导意义。
Objective In order to study the epidemiological survey of hospital infection from the sphere of biology to that of molecular biology.Methods chromosomal DNA of Pseudomonas aeruginosa isolated from a hospital infection were digested with BamHI,HindⅢ and EcoRI respectively,and then to get the REA spectrum by gel electrophoresis.With PCR technology,16s and 23s ribosomal RNA genes were amplified from E.coli,labelled by[α-P_(32)]dATP with nick translation and applied as broad spectrum probes and then hybridized with Southern hybridization to gain the rDNA fingerprinting. Results The results demonstrated that in the same hospital,the REA and rDNA fingerprintings of bacteria is olated from different patients of various wards and environments are different,but those from patients of the same ward are quite the same.Hospital infections are related with environment pollution. Conclusions This method can play an important role in bacterial classification,exact identification of infective sources,blocking infective routes,controlling and preventing hospital infections.
基金
山东省青年科研基金资助
