摘要
目的了解人类细胞中线粒体的肌酸激酶(CKmt)是否存在亚型。方法首先提纯人类心肌组织的 CKmt,并通过免疫大白兔得到了兔抗人 CKmt 抗体,然后用薄层等电聚焦法(IEF)对提纯的人心肌组织 CKmt 进行分析。结果 CKmt 可进一步分为三种亚型,将向阳极泳动最快的区带称为CKmt-1,次之的为 CKmt-2,泳动最慢的区带为 CKmt-3,他们的等电点分别为6.86、6.98和7.05。结论在正常人心肌组织细胞的线粒体中,存在三种 CKmt 亚型,CKmt-3为其主要形式,如果将CKmt-3置于正常人低 CK 活性血清中,则可能出现 CKmt-3→CKmt-2→CKmt-1的转化趋势。
Objective To know whether human mitochondrial creatine kinase has its own iso- forms.Methords Mitochondrial creatine kinase(CKmt)was isolated from normal human heart tissue, and rabbit antiserum specfic for CKmt showed no coss-reactivity to cytosolic creatine kinase(CK).Re- sults Human heart CKmt was present in three isoforms by thin isoelectric focusing(IEF)examina- tion,and two isoforms had mobility more cathodic than CK-MM_3.The fast-moving cathodal isoform and the slow-moving cathodal isoform were designated as CKmt-3 and CKmt-2,respectively.The iso- forms which showed an electrophoretic migration pattern similar to CK-MM3 was CKmt-1.The pI of CKmt isoforms are 7.05,6.98,and 6.86 respectively.Conclusions The mitochondrial creatine kinase exists in human heart tissue of three isoforms.We speculated that CKmt-3,which is the major form of CKmt isoforms,could be converted into CKmt-2,and finally into CKmt-1 in normal human serum.
关键词
线粒体
肌酸激酶
等电聚
心肌梗塞
Creatine kinase Isoelectric focusing Myocardial infarction
