摘要
目的：采用特异性检测方法，定量测定卵巢癌患者血清胎盘型碱性磷酸酶（ＰＬＡＰ），为临床可疑患者提供诊断依据。方法：以ＰＬＡＰ为抗原，制得兔抗ＰＬＡＰ抗体，依据抗体免疫特性建立特异的定量检测ＰＬＡＰ固相直接免疫测定法。采用此方法测定８６例健康对照者及８１例恶性肿瘤患者血清ＰＬＡＰ。结果：对照者血清ＰＬＡＰ活性呈偏态分布，第９５百分位数的上限为０．３８Ｕ／Ｌ，以该值作为正常与升高的分界值，恶性肿瘤患者中１８例血清ＰＬＡＰ升高，阳性率为２２．２％，其中卵巢癌患者阳性率为６１．９％。结论：应用固相直接免疫法检测ＰＬＡＰ，对卵巢癌诊断有一定意义。
Objective:Toestablishamethodfordiagnosisofmalignanttumorsbydeterminationoftheplacentalalkalinephosphatase(PLAP)inserum.Methods:AsolidphaseenzymeimmunoasaywasadopatedbyanantibodyagainstPLAP.PLAPinserumof81patientswithmalignanttumorand86healthypeopleascontrolweredetermined.Results:Inthecontrolgroup,thePLAPactivityshoweddeviativedistribution,the95thpercentilevaluesofPLAPincontrolgroupwas3.8U/L.Ifthisvalueservedasnormalcut-ofline,18casesamong81patientswithmalignanttumorswerepositive.Thepo-sitiveratewas22.2%,butthepositiveratesinovariancancerwas61.9%.Conclusions:TheincreasingofserumPLAPmaybehelpfulforthediagnosisofmalignanttumorsespecialyovariancancer.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
1996年第2期107-109,共3页
Chinese Journal of Obstetrics and Gynecology
