摘要
用互补于幽门螺杆菌（ＨＰ）脲酶Ｃ基因序列的两组寡核苷酸引物，建立了检测ＨＰ的套式聚合酶链反应方法，两轮ＰＣＲ扩增分别产生６６４ｂｐ和３０１ｂｐ的特异ＤＮＡ片段，灵敏度分别可达１００ｆｇ和１ｆｇ细菌ＤＮＡ。此方法可快速、灵敏、特异地检出各种临床样品中的ＨＰ，是一种非常有用的分子流行病学研究工具。
A nested polymerase chain reaction(PCR)method for detecting Helicobacter pylori(HP)was developed by using two sets of primers based on the nucleotide sequences of the Ure C gene of HP. PCR by lst step primers produced a specific 664 bp DNA band and 2nd step primers 301 bp DNA band,The first and second step PCR achieved the sensitivity as small as 100 fg and 1 fg of target DNA,respectively.We demonstrated that this nested PCR assay was highly sensitive,specific,rapid and simple,and that it was able to detect HP in various clinical materials.It provides a useful tool for molecular epidemiological research of HP.
出处
《临床检验杂志》
CAS
CSCD
北大核心
1996年第1期11-13,共3页
Chinese Journal of Clinical Laboratory Science
基金
铁道部科技司资助
