摘要
目的:探讨二氧化硅(SiO2)致小鼠肝脏局部纤维化的方法及其可能的机制。方法:C57BL/6J小鼠24只随机分成实验对照组(生理盐水)、碘油组(超液化碘油)、混和液组(超液化碘油+SiO2),分别用生理盐水、超液化碘油及混和液各0.2 mL局部注射小鼠右肝,3个月后观察注射局部病理变化并分别统计比较变性坏死、纤维化及巨噬细胞和纤维化结节的面积差异,测量血清免疫球蛋白G(IgG)、血清免疫球蛋白M(IgM)变化。结果:3组动物注射局部处肉眼观察无明显差别;镜下观察,3组小鼠注射局部变性坏死面积不同,其中混和液组引起局部变性坏死面积最大(χ2=15.4,P<0.01);混和液组与碘油组两两比较变性坏死面积差异无显著性意义(Z=-0.8,P>0.05);在混和液组可见肝脏组织局部的巨噬细胞结节和纤维细胞增生,局部纤维细胞增生较碘油组差异有显著性意义(Z=-2.5,P<0.05);3组小鼠血清IgM水平均数不同(F=1 720.3,P<0.01),其中,混合液组最高(314.7±10.7)mg/L。结论:SiO2与超液化碘油的联合应用可以显著地引起小鼠肝脏局部纤维细胞增生,其机制可能与体液免疫因素IgM水平的变化有关。
Aim： To find out the way and the mechanism of the local fibrosis of the mouse liver induced by SiO2（silicon dioxide）. Methods： 24 C57BL/6J mice were distributed randomly into control group （normal salin）, lipiodol group （ultra - liquefied lipiodol） and mixture group （ultra - liquefied lipiodol ± SiO2） and injected respectively with 0.2 mL normal salin, ultra - liquefied lipiodol and mixture, the pathologic changes of local livers injected and the serum immune globulin G （IgG） and immune globulin M （IgM） were observed after three months. Results： No difference in macroscopic shape among three groups, however, the areaof degeneration and necrosis were showed significant difference, especially in the mixture group （X^2 = 15.4, P 〈 0.01 ）. We did not find the significant difference between the mixture group and lipiodol group （ Z = - 0.8, P 〉 0.05 ） ; The formation of macrophage nodes, fibrocyte nodes and the fibrocyte proliferation of the mouse livers in mixture group were more significant than in lipiodol group （Z = -2.5, P 〈 0.05）. the serum concentration （314.7 ± 10.7 mg/L） of IgM in the mixture group was the highest （ F = 1720.3, P 〈 0.01 ）. Conclusion： The coapplication of SiO2 and lipiodol can cause the local fibrocyte proliferation of mouse liver signifi- candy, and the concentration change of IgM may be associated with this process.
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
北大核心
2005年第4期491-494,507,共5页
Journal of Jinan University(Natural Science & Medicine Edition)
