摘要
目的:建立多重PCR方法并对临床菌株进行扩增及整合子分类。方法:采用多重PCR对21株来自临床的耐药菌株进行了第一、二和三类整合酶基因(intI)筛选。结果:16株携带第一类整合酶基因;1株携带第一和第二类整合酶基因;2株含第二类整合酶基因;1株含第三类整合酶基因;1株不含第一、二和三类整合酶基因。结论:筛选细菌耐药整合子基因分类的多重PCR方法简便可靠;整合子广泛存在于临床耐药细菌中。
Aim： To construct a multi - PCR assay using specific primers of intl, int2 and int3 to screen class 1,2 and 3 integrase （intⅠ） among clinical samples. Methods： of 21 strains isolated from clinical source were detected by multi - PCR in this study. Results： Among 21 strains, 16 strains were shown harbored /ntl. Of 1 strain was found to carry intl and int2 genes. Of 2 strains were positive for int2 gene. Of 1 strain was positive for int3 gene. Of 1 strain was negative in multi - PCR. Conclusion： A multi - PCR assay to screen class 1,2 and 3 integron at one time was constructed and confirmed to be practicable. The result shows that integrons have widely existed in antibiotic resistance clinical isolates of bacteria.
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
北大核心
2005年第4期480-485,共6页
Journal of Jinan University(Natural Science & Medicine Edition)
基金
广东省自然科学基金项目(04020050)
广州市科技计划项目(2004J1-C0161)
