摘要
目的研究17β-雌二醇对涎腺黏液表皮样癌高转移细胞Mc3黏附、侵袭和移动力的作用。方法采用细胞体外黏附实验、体外移动实验、化学趋化性实验以及明胶底物SDS-PAGE凝胶电泳对基质金属蛋白酶(MMP)活性测定的方法研究17β-雌二醇对Mc3细胞黏附、侵袭和移动力可能存在的作用,用免疫组化的方法检测Mc3细胞的雌激素受体的表达。结果不同浓度(10-9、10-8、10-7、10-6mol/L)的17β-雌二醇处理Mc3细胞72h后,其黏附率分别为38·3%、50·4%、69·2%、91·1%;而对照组为25·0%;作用48h后,10-9、10-8、10-7、10-6mol/L的17β-雌二醇对Mc3细胞在纤维连接蛋白(FN)上的移动促进率分别为16·9%、40·9%、36·4%、38·8%。在10-6mol/L的17β-雌二醇的作用下,其对FN的化学趋化性增加60·3%。10-9、10-8、10-7、10-6mol/L的17β-雌二醇均可不同程度的促进Mc3细胞基质金属蛋白酶(MMP-2)的活性,在10-6mol/L时尤为显著。在Mc3细胞中有雌激素核受体的表达。结论生理浓度的雌激素可促进Mc3细胞的黏附、侵袭和移动力。
Objective To study the effects of 17 beta-estradiol on the adhesion, invasion and motility potential of salivary mucoepidermoid carcinoma Mc3 cells. Methods The effects of 17 betaestradiol on adhesion, invasion and motility potential of salivary mucoepidermoid carcinoma Mc3 cells were investigated with cell attachment assay on fibronectin （ FN）, wound assay, chemotaxis assay, and gelatinincorporated SDS-PAGE electrophoresis. The expression of estrogen receptor in Mc3 cells was determined by immunohistochemistry assay. Results Attachment rates of Mc3 cells treated with E2 at 10^-9 , 10^-8, 10^-7, 10^-6 moL/L were 38. 3% , 50.4% , 69. 2% and 91.1% respectively, and the rate in control was 25.0%. When exposed to 17 beta-estradiol at 10^-9,10^-8, 10^-7 and 10^-6 moL/L for 48 h, motility of Mc3 cells on FN increased by 16. 9% , 40. 9% , 36. 4% and 38.8% respectively. When at 10^-6 moL/l, 17 beta-estradiol increased chemotaxis potential of Mc3 cells to FN by 60. 3%. The activity of 68 000 matrix metalloproteinase （MMP-2） of Mc3 cells was enhanced at different levels by 10^-9, 10^-8, 10^-7, 10^-6 moL/L of 17 betaestradiol, and estrogen receptor was also detected in nucleus of Mc3 cells by immunohistochemistry assay. Conclusions 17 beta-estradiol at physiological concentration may enhance the adhesion, invasion and motility potential of salivary mucoepidermoid carcinoma Mc3 cells.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2005年第5期390-393,共4页
Chinese Journal of Stomatology
