摘要
本研究采用ＤＮＡ重组技术及ＰＣＲ方法对人甲胎蛋白（ＡＦＰ）基因顺式调控元件＋２９ｂｐ至－５．１ｋｂ区进行改造，将改建的ＤＮＡ片段分别克隆到荧光素酶报告基因载体ｐＧＬ２－Ｂａｓｉｃ中，构建了６种含有ＡＦＰ基因增强子和／或沉寂子不同组合的载体。以期筛选出对人肝癌细胞特异的，具有强启动活性的ＡＦＰ顺式调控元件组合，为进一步实行肝癌基因治疗提供依据。
sing recombinant DNA techniques and PCR method.we have arneliorated the 5.1 kb 5'- flanking region of the human a-fetoprotein(AFP)gene containing transcription control elements with characteristics of enhancers and silencers.and got six DNA fragments of different length that were cloned into luciferase expression vector pGL2-Basic.Hepatoma-specific transcription-regulatory sequences of the human AFP gene were expected to be obtained.
出处
《基础医学与临床》
CSCD
1996年第1期31-38,共8页
Basic and Clinical Medicine
基金
国家教委博士点基金
关键词
肝脏肿瘤
甲胎蛋白
DNA重组
AFP gene cis-regulatory element hepatocellulr carcinoma luciferse reporer gene Dept.of Biochemistry.Institute of Basic Medicine.Chinese Academy of Medical Sciences.BeiJing 100005
