摘要
目的探讨SW872前脂肪细胞分化过程中脂联素受体基因表达的变化。方法体外培养,0.6 mmol/L油酸诱导SW872前脂肪细胞分化,油红O染色观察细胞内脂肪的聚积;RT-PCR方法检测不同分化时间点脂联素受体(AdipoR)1 mRNA及AdipoR 2 mRNA水平。结果0.6 mmol/L油酸诱导分化72 h,能成功将SW872前脂肪细胞分化为成熟脂肪细胞;SW872前脂肪细胞表达两种脂联素受体,诱导分化刺激48 h,AdipoR 1 mRNA水平是诱导分化前的2.16倍,AdipoR 2 mRNA水平是诱导分化前的2.34倍;诱导分化72 h,AdipoR 1 mRNA水平是诱导分化前的2.54倍,AdipoR 2 mRNA水平是诱导分化前的4.09倍。结论SW872脂肪细胞表达脂联素两种受体增殖,且两种AdipoR的基因表达呈分化相关性。
Objective To explore genes expression of adiponectin receptors during differentiation of SW872 preadipocytes. Methods SW872 preadigocytes were cultured in vitro and induced to differentiate by 0.6mmol/L oleic acid. During the progress of differentiation, the morphological changes of SW872 cells were observed and the differentiation rate was assayed by oil-red O staining. Adiponectin receptors mRNA was measured by reverse transcription-polymerase chain reaction during differentiation of SW872 preadipocytes. Results 1. After stimulated by 0.6mmol/L oleic acid for 72 hours, almost all SW872 cells were differentiated, and there were lots of fat droplets in the cells. 2. There were adiponectin receptors genes expressions in SW872 preadipocytes. After 72 hours,and the levels of adiponectin receptor（AdipoR） 1 nRNA and AdipoR 2 mRNA were markedly increased up to 2.54 and 4.09 times, respectively. Conclusion There are AdipoRl and AdipoR2 genes expressions in fat cells and the expressions are differentiation dependent.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2005年第9期891-893,共3页
Journal of Applied Clinical Pediatrics
基金
国家自然科学基金项目资助(30470645)
关键词
脂肪细胞
油酸
细胞分化
脂联素受体
fat cell
oleic acid
cell differentiation
adiponectin receptors
