摘要
四种叶形吸虫成虫初级精母细胞核ＤＮＡ含量测定表明，寄生于较高等鱼类宿主的中华叶形吸虫和鳗鲡叶形吸虫的ＤＮＡ含量较高（Ｃ值分别为０．２５ｐｇ和０．２６ｐｇ），而寄生于较低等鱼类宿主的鲶叶形吸虫和巴氏叶形吸虫的ＤＮＡ含量较低（Ｃ值分别为０．２１ｐｇ和０．２０ｐｇ）。这四种叶形吸虫成虫全蛋白质浓度梯度凝胶电泳分析进一步显示，中华叶形吸虫与鳗鲡叶形吸虫构成一近缘亚群（Ｓｍ＝０．６５７９，Ｄ＝０．４１８７），而鲶叶形吸虫和巴氏叶形吸虫构成另一近缘亚群（Ｓｍ＝０．６１７６，Ｄ＝０．４８１９）；这两个亚群间的相似系数值（Ｓｍ）小于０．５３，遗传距离值（Ｄ）大于０．６４。以上结果从分子水平上再次证实将叶形吸虫属划分为两个亚属是有根据的。
The DNA contents of the four species in Phyllodistomum are measured withfluorescence microphotometry after Feulgan staining.P.sinense and P.anguilae have a higherDNA content(0.25 and 0.26 pg respectively);P.parasiluri and P.pawlovskii have a lowerDNA content(0.21 and 0.20 pg respectively).In the light of evolutionary theory it is suggested that the former two species are evolutionarily advanced than the latter two species.Thepatterns of general protein are analysed by means of gel concentration gradient electrophoresis. The result also indicates that P.sinense and P.anguilae constitute a related subgroup(Sm = 0.6579 and D = 0.4187);P.parasiluri and P.pewlovskii constitute another related subgroup(Sm = 0.6176 and D = 0.4819).There is a genetic difference between the two subgroup(Sm<0.53 and D>0.64) The present study provides the evidence at molecular level for dividing the genus Phyllodistomum into two subgenera.
出处
《中国兽医寄生虫病》
1996年第1期1-4,共4页
Chinese Journal of Veterinary Parasitology
基金
国家自然科学基金
