摘要
目的:通过序列比对分析SEN病毒(SENV)不同开放阅读框(ORF)基因系统进化情况。方法:用聚合酶链反应(PCR)方法扩增武汉地区SENVORF1和ORF2基因,结合GenBank中SEN病毒基因信息对该基因进行序列测定、并采用clutal软件系统进行分析。结果:武汉SEN病毒株与北京株同源性较高,与日本株有一定的差异,和TT病毒有较大差异。结论:SENV可能不是TTV亲缘关系较远的变异株,而是一群不完全相同成员组成的病毒种。
Objective： To phylogenetic analysis of different open reading frame （OFR） of SEN virus, Methods： Polymerase reaction products of SENV were sequenced and phylognetic analysis with clutal computer soft according to sequences on Genebank. Results： The homololgy of nuclcotide sequence between Wuhan isolates and Bejing isolate were semblable, had some difference between Japan isolate and much different with TT virus. Conclusion： SEN virus, belong to the Circoviridae family, may be different strain from TT virus.
出处
《中西医结合肝病杂志》
CAS
2005年第4期212-213,217,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
湖北省科委资助项目(No.2002AA301C32)
