摘要
目的研究姜黄素对NB4细胞组蛋白H3和非组蛋白P53的乙酰化和细胞增殖的作用,探讨姜黄素抗白血病机制。方法应用不同浓度(50,25,12.5,6.25,3.125μmol/L)的姜黄素作用NB4细胞不同时间(0,4,8,12,24h),MTT法测定姜黄素对NB4细胞增殖的影响,Western blot法检测乙酰化组蛋白H3和乙酰化P53的水平。结果姜黄素以时间和剂量依赖方式抑制NB4细胞增殖,在24h和36h的IC50值分别为40μmol/L和25μmol/L;能明显上调组蛋白H3的乙酰化水平,促进P53的表达和P53的乙酰化。结论姜黄素具有去乙酰化酶抑制剂作用,能上调组蛋白H3乙酰化水平,促进肿瘤抑制因子P53表达和活化,抑制白血病细胞增殖。
Objective To investigate the effects of eurcumin on the acetylation of histone H3, P53 and the proliferation of NB4 cells. Methods The total protein was extracted from NB4 cells treated without or with different concentrations of cureumin （ 50, 25, 12.5, 6.25 and 3. 125 μmol/L ） for different time （0, 4, 8, 12, 24 h）. Western blot analysis was performed to determine the levels of acetylated histone H3, P53 and aeetylated P53. MTT assay was performed to examine the growth inhibition effect of eurcumin on NB4 cells. Results Curcumin could inhibit the proliferation of NB4 cells in a time- and dose- dependent manner, with the 1C5o at 24 h and 36 h of 40 μmol/L and 25 μmol/L, respectively. The levels of histone H3 acetylation, P53 expression and P53 aeetylation were inereased obviously. Conclusion Curcumin functions as a deaeetylase inhibitor,which could increase the level of aeetylated histone H3, enhance the expression and activity of tumor suppressor P53, and inhibit the proliferation of NB4 cells.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2005年第9期551-553,共3页
Chinese Journal of Hematology
基金
国家自然科学基金资助项目(30271672)
