摘要
目的探讨大白兔颈动脉供体不同处理方法移植后血管形态学变化。方法建立大白兔颈动脉移植模型(新西兰兔:颈动脉供体,日本兔:宿主),根据移植前供体血管不同处理方法,随机分为A(自体移植组)、B(新鲜组)、C(青霉素和链霉素处理后常温保存组)、D(青霉素和链霉素处理后冷冻保存组)4组。光镜下观察移植后1、2、3周血管形态学改变。结果A组血管结构正常,仅有炎性细胞浸润。其余各组均以移植血管段远端腔内血栓形成、近端通畅为主要表现。镜下改变以第3周最典型,B组可见血管结构紊乱,管腔内有混合性血栓,机化后侵袭血管壁,内膜变性、增生、中膜维素性坏死,外膜大量炎性细胞浸润;C组血管结构大致正常,管腔有红色血栓,内膜完整,中膜、外膜大量炎性细胞浸润;D组血管结构正常,管腔血栓机化再通,中膜、外膜大量炎性细胞浸润。结论青霉素和链霉素等离体预处理措施可降低供体血管抗原性;液氮冷冻保存可能具有协同效应。
[ Objective ] To investigate the morphological changes of carotids pretreated by different methods and transplanted between New Zealand （donor） and Japanese （recipient） homozygotic male rabbits. [Methods] Establishing rabbits＇ carotid transplantation model with autograft （Group A）, or allograft carotids. The allograft implanted without any intervening （Group B）, or preconditioned by antibiotics （Penicillin/Streptomycin）, preserved under room temperature （Group C） or cryopreserved in liquid nitrogen （Group D）. The gross and microscopic changes were one-blinded observed under light microscope. [Results] The vascular structure of autografts in Group A altered the least in all the trial groups and remained almost normal, we could only find some inflammatory cells infiltration. The allografts mainly manifested thrombosis in the distance ends with unobstructed proximate ends. The typical microscopic vascular morphologies altered utmost obvious in the vessels harvested at 1st, 2nd, and the 3rd week, particularly those in Group B, such as intima degeneration and hyperplasia, media fibroplasias and necrosis, gross inflammatory cell infiltration, and reopening of lumina after the organization of mixing thrombus. The structures of allografts in Group C seemed next to normal, aggregating with much luminal red thrombus, intact intima, and much gross inflammatory cell infiltrations in media and envelope. The morphological alterations in allografts in Group D varied relatively the same as in Group A, the vessels reopened after thrombus＇ organization, and also with much gross lymphocyte infiltration. [Conclusion] Allografts＇ antigenicity may be down-regulated by exposing to in vitro preconditioning techniques involving with combination of penicillin and streptomycin. Further more, cryopreservation may have synergetic effect.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2005年第16期2416-2420,共5页
China Journal of Modern Medicine
关键词
颈动脉
移植
排斥反应
形态学
carotid
transplantation
rejection
morphology
