摘要
通过搅拌式超滤器对牛初乳中的IGF-1超滤粗提,制备IGF-1的粗提物.对超滤条件的研究发现,在pH值为8.0碱性条件下的IGF-1回收率比在pH值为4.2酸性条件下高.将超滤后的粗提物分别用SDS-PAGE电泳检测,来验证每步骤的提纯效果.再将浓缩后的IGF-1粗提物用葡聚糖G-200柱层析进一步分离纯化,得到IGF-1纯品.每一步骤的IGF-1质量浓度利用放射免疫测定法(RIA)测定.
The primitive IGF-1 from colostrums was obtained by ultrafiltration, and a higher yields could be obtained at pH 8.0 other than pH 4.2. The rude IGF-1 after ultrafiltration was tested by SDS-PAGE, and the pure IGF-1 was obtained Chromatography with a Sephadex G-200 column. IGP-I density in each process was assayed through Radio Immunition Assay（RIA）.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2005年第4期73-75,80,共4页
Journal of Food Science and Biotechnology
关键词
牛初乳
胰岛素样生长因子-1
提取和纯化
超滤
colostrum
insulin-like growth factor-1
separation and purification
diafiltration
