摘要
目的观察丙泊酚对大鼠海马突触体谷氨酸和γ氨基丁酸(GABA)Ca2+依赖性释放和非Ca2+依赖性释放的影响。方法制备突触体后用人工脑脊液(aCSF)孵育,分为7组,应用丙泊酚(propofol)的浓度分别为3(Pro3组)、10(Pro10组)、30(Pro30组)、100(Pro100组)和300μmol·L-1(Pro300组),脂肪乳剂对照组加入脂肪乳(Intralipid组),空白对照组(Control组)不加入任何药物。在观察Ca2+依赖性释放时,加入二氢海人藻酸和哌啶酸;在观察非Ca2+依赖性释放的时候,去除aCSF中的Ca2+。在37℃的条件下,应用20μmol·L-1藜芦定或30mmol·L-1KCl诱发递质释放。应用反相高效液相色谱法测定aCSF中谷氨酸和GABA的浓度。结果①Ca2+依赖性递质释放:应用藜芦定时,Pro30、Pro100和Pro300组的谷氨酸和GABA释放量低于Intralipid组(P<0.01或P<0.05);应用KCl时,各组的谷氨酸和GABA释放量无差异(P>0.05)。②非Ca2+依赖性释放:各组应用藜芦定和KCl诱发的谷氨酸和GABA释放量无差异(P>0.05)。结论丙泊酚可以抑制Ca2+依赖性谷氨酸和GABA的释放,而对非Ca2+依赖性谷氨酸和GABA释放无影响。
AimTo investigate the effect of propofol on the release of glutamate and γ-aminobutyric acid （GABA） from rat hippocampal synaptosomes. Methods Synaptosomes were made from hippocampus and incubated with artificial cerebrospinal fluid （aCSF）. When Ca^2＋-dependent release of glutamate and GABA were observed, dihydrokainic acid （DHK） and nipectic acid were added into aCSF. When Ca^2＋-independent release of glutamate and GABA were observed, Ca^2＋ was omitted from aCSF. The release of glutamate and GABA were measured using high-performance liquid chromatograph （HPLC）. Results ①30,100 and 300μmol ·L^-1 propofol significantly inhibited veratridineevoked Ca^2＋-dependent release of glutamate and GABA （P 〈 0. 01 or P 〈 0.05 ）. However, propofol has no effect on elevated KCl-evoked Ca^2＋-dependent release of glutamate and GABA （ P 〉 0. 05 ）. ②Veratridine- or elevated KCl-evoked Ca^2＋ -independent releaseof glutamate and GABA was not affected significantly by propofol （P 〉 0.05 ）. Conclusion Propofol could inhibit Ca^2＋-dependent release of glutamate and GABA. However, it has no effect on the Ca^2＋-independ- ent release of glutamate and GABA.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2005年第8期935-939,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No39370660)
江苏省教育厅开放基金资助项目(NoKJS03084)
关键词
丙泊酚
海马
突触体
谷氨酸
Γ-氨基丁酸
propofol
hippocampus
synaptosome
glu tamate
γ-aminobutyric acid
