阿托伐他汀对人内皮细胞间粘附分子-1表达的影响被引量：7

The Effects of Atorvastatin Treatment on Oxidized Low-Density Lipoproteins Induced Expression of ICAM-1 Mrna in Cultured Human Umbilical Vein Endothelial Cells

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摘要目的:探讨不同浓度的阿托伐他汀(atorvastatin)对氧化低密度脂蛋白(ox-LDL)诱导的人脐静脉内皮(HUVECs)细胞间黏附分子-1(ICAM-1)mRNA表达的影响.方法:体外培养人脐静脉内皮细胞,待细胞生长到融合状态时加入ox-LDL(100μg/ml),作用24小时后分别加入不同浓度的阿托伐他汀(1～30μmol/L),采用逆转录聚合酶链反应(RT-PCR)技术测定ICAM-1 mRNA的表达.结果:Ox-LDL可诱导ICAM-1 mRNA的表达,不同浓度的阿托伐他汀可抑制ox-LDL诱导的ICAM-1 mRNA的表达,并呈浓度依赖性(P<0.05).结论:阿托伐他汀可抑制ox-LDL诱导的HUVECsICAM-1 mRNA的表达,并呈浓度依赖性. Objective： This study was to investigate the effects of atorvastatin treatment on intereelluar adhesion molecule - 1 （ICAM - 1 ）, in cultured human umbilical vein endothelial cells （HUVECs）. Methods： HUVECs were cultured in endothelial basal medium.Cells were grown to confluence and used for oxidized low - density lipoproteins （ox - LDL） .After 24 hours,HUVECs were cocultured with the ox - LDL and atorvastatin（ 1 - 30μmol/L） for another 24 hours. Expression of ICAM - 1 mRNA were determinded by reverse transcription - polymeras chain reaction （RT - PCR） . Results： Expression of ICAM - 1 mRNA were detected in ox - LDL - induced HUVECs. Atorvastatin dose - dependently （ 1 - 30μmol/L） decreased the ctpression of ICAM - 1 mRNA in ox - LDL- induced HUVECs, P 〈 0.05. Conclusion： Atorvastatin decreases dose- dependently the expression of ICAM- 1 mRNA in ox - LDL- induced HUVECs. Atorvastatin has anti - inflammatory effects which are probabiy related to reducing effect of ICAM - 1.

作者庞文跃姜立清胡大一

机构地区中国医科大学附属第二医院心内科首都医科大学附属北京同仁医院心血管疾病诊疗中心

出处《中国医药导刊》 2005年第4期295-297,共3页 Chinese Journal of Medicinal Guide

关键词阿托伐他汀内皮细胞间黏附分子-1 基因表达氧化低密度脂蛋白脐静脉炎症动脉粥样硬化 Oxidized low-density lipoproteins Human umbilical vein endothelial cells Intercelluar adhesion molecule-1 Atorvastadn

分类号 R965 [医药卫生—药理学]

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