摘要
以获取高营养性的蚯蚓含铁蛋白质为目的,根据蚯蚓体内血红蛋白分子结构的特性与分布状态,采用了双水相萃取等分离纯化工艺进行了制备生物态含铁蛋白质纯品的研究。结果表明,用聚乙二醇(12%,W/W)-硫酸铵(12%,W/W)组成的双水相萃取样品后直接用等电点沉淀法,即在pH约为5.1的缓冲液中进行透析可获得高纯度的蚯蚓含铁蛋白。在此基础上若再采用QSepharosefastflow离子交换层析和梯度洗脱的方法就能使产品的质量进一步提高。综合分析说明了研究中所采用的工艺较简单,成本不高,可操作性好,且能获得高品质的含铁蛋白质产品。研究为从食品层面上利用开发蚯蚓、推动饲养蚯蚓产业的发展和制备补血功能食品等提供了实验数据。
Aim at obtaining iron-containing protein from earthworm as nutrient, aqueous two-phase extraction and other methods were performed in purification process based on the characters and their exist state of hemoglobin in earthworm body. Results showed that using directly isoelectric point precipitation at pH 5.1 after the aqueous two-phase extraction composed of 12%（W/W）polyethylene glycol and 12%（W/W）ammonium sulfate may separate well the iron-containing protein from other substances. Addition of the ion exchange chromatography Q Sepharose fast flow column in final process may get pure Lumbyicus terrestris hemoglobin. The preparation of iron-containing protein product in high quality from earthworm was simple, performed easily with lower cost based on this study, which was very useful for manufacture blood tonic food utilizing earthworm as materials.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第8期170-173,共4页
Food Science
关键词
蚯蚓
含铁蛋白质
纯化
双水相萃取
Earthworm
iron-containing protein
purification
aqueous two-phase extraction
