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柑橘衰退病毒柚分离株的p25/Hinf1 RFLP分析 被引量:5

A p25/Hinf1 RFLP analysis of the Citrus tristeza virus isolates infecting pummelo
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摘要 对123个柑橘衰退病毒柚分离株进行p25/Hinf1 RFLP分析明确:样品中,单一p25/Hinf1 RFLP组群样品占样品总量的82.9%,说明我国田间受侵染柚类中柑橘衰退病毒株系相对较为单一;RFLP组群6的样品占样品总量的79.7%,说明目前柚类生产上的优势流行株属于p25/Hinf1 RFLP组群6,初步认定为强毒株系;柑橘衰退病毒株柚分离株S102、S104、S106、S108属于p25/Hinf1 RFLP组群5,初步认定是弱毒株系。 Citrus Tristeza Virus (CTV) is one of the most economically important pathogen of citrus in many citrus producing areas of the world. Pummelo dwarf caused by CTV has become a serious problem in China recently. In order to discover their pathogenic characteristics, 123 CTV isolates infecting pummelo were studied by p25/Hinf1 RFLP assay. Single p25/Hinf1 RFLP groups were detected in 82.9% of the 123 samples, and the dominant group was p25/Hinf1 RFLP group 6,which accounted for 79.7% of the total. There were 4 isolates belonging to p25/Hinf1 RFLP group 5,which could be mild CTV strains and should be further studied.
出处 《植物保护》 CAS CSCD 北大核心 2005年第4期28-31,共4页 Plant Protection
基金 留学回国人员科技活动择优资助项目"柑橘茎陷点型衰退病防治技术研究"。
关键词 植物病毒学 柑橘衰退病毒 分离株 限制性片段长度多态性分析 phytovirology Citrus Tristeza Virus isolate RFLP assay
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