摘要
目的:研究缬沙坦对大鼠心肌梗死(MI)后电压门控钾通道(Kv)9.X(Kv9.1、Kv9.2、Kv9.3)变化的影响。方法:通过结扎大鼠冠状动脉左前降支近端建立MI大鼠模型,手术后存活大鼠随机分为MI组(7、30d)和缬沙坦组(7、30d:缬沙坦30mg/kg,qd),同时设立相应假手术组(7、30d)。MI组和假手术组对应给予等量0.85%氯化钠溶液。应用半定量RT-PCR方法检测非梗死左室心肌Kv9.1、Kv9.2、Kv9.3mRNA量。结果:缬沙坦组与MI组比较,无论7d或是30d,Kv9.1、Kv9.2、Kv9.3mRNA量均明显升高(P<0.05或P<0.01);与假手术组比较,无论7d或是30d,Kv9.1、Kv9.2、Kv9.3mRNA量差异均无统计学意义;MI组与假手术组比较,无论7d或是30d,Kv9.1、Kv9.2、Kv9.3mRNA量均明显降低(P<0.05或P<0.01);MI30d时与7d时比较Kv9.1、Kv9.2、Kv9.3mRNA量下降(均P<0.05)。结论:缬沙坦可以显著逆转MI后Kv9.1、Kv9.2、Kv9.3mRNA表达的下调,且该作用迅速而完全。
Objective：To study the effects of valsartan on the expression of potassium channels Kv9. X （Kv9. 1 .Kv9. 2.Kv9. 3） of left ventricular non infarcted myocardiums in post-myocardial infarction （post MI） rats. Method： Using a rat model of MI, induced by the left anterior descending coronary artery （LAD） ligation in female Sprague-Dawley rats, the living rats randomly divided into post MI group （ 7 days group n =10 ; 30 days group n= 11） or valsartan group（7 days group n =10： 30 days group n =10; valsartan 30 mg · kg^-1· day^-1）. Accord ingly, the sham-operation group （sham- group） was established. Using semi-quantitative reverse transcriptase-polymerase chain reaction （RT-PCR）, we measured Kv9. 1,Kv9. 2,Kv9.3 mRNA in each group. Result：Compared to MI groups, potassium channels Kv9.1 ,Kv9. 2 ,Kv9. 3 mRNA in the valsartan groups remarkably increases regardless of on 7 days or 30 days （respectively, P〈0.05; P〈0.01）. There is no difference between the valsartan groups and the sham-groups. Compared to the sham-groups, potassium channels Kv9.1,Kv9.2,Kv9.3 mRNA in the MI groups remarkably decreases regardless of on 7 days or 30 days （respectively, P〈0.05; P 〈0.01）. Compared to on 7 days after myocardial infarction, potassium channels Kv9. 1,Kv9. 2,Kv9.3 mRNA remarkably decreases on 30 days （P〈0.05）. Conclusion：Valsartan may reverse the downregulation of expression of potassium channels Kv9.1 ,Kv9.2,Kv9.3 mRNA in post-myocardial infarction rat markedly, which is quick and absolute.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2005年第8期485-487,共3页
Journal of Clinical Cardiology
关键词
心肌梗死
钾通道
缬沙坦
Myocardial infarction
Potassium channel, Kv9. X
Valsartan
