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凋亡蛋白对Hep2细胞影响的研究

Influence of Apoptin on Hep2 cell lines
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摘要 从鸡贫血病毒(CAV———ch icken anem ia virus)总DNA中克隆凋亡蛋白(Apoptin)基因片段,并与pcDNA3.1/CT-GFP-TOPO载体构建融合载体,转化E.coli.DH5α,筛选氨苄抗性阳性克隆并鉴定.以该融合载体转染Hep2细胞后,在倒置荧光显微镜下观察到绿色荧光标记,同时观察到细胞核形态学呈凋亡特征变化,表明凋亡蛋白可以诱导Hep2细胞系发生凋亡. The pcDNA3.1/CT- GFP- Apoptin- TOPO was reconstructed with apoptin gene cloned from all DNA of chicken anemia virus. The anti-ampicillin cloney was selected after transforming into E. coli DH5α, then the plasmid was verified and purified. Hep2 cell lines were transfected by the fusion vectors, and GFP marker was observed by inverted fluorescence microscope. Subseq as exclusive apoptotic description. Theses implied that Hep2 uently, the morphology was changed as same lines were apoptosis induced by apoptin.
作者 国泰 杨谦 高洪雷 孙?晗 王笑梅
机构地区 哈尔滨工业大学理学院 中国农业科学院哈尔滨兽医研究所
出处 《哈尔滨工业大学学报》 EI CAS CSCD 北大核心 2005年第8期1066-1067,1073,共3页 Journal of Harbin Institute of Technology
基金 黑龙江省自然科学基金资助项目(C203)
关键词 凋亡蛋白 细胞凋亡 Hep2传代细胞 apoptin apoptosis Hep2 cell lines
分类号 Q2 [生物学—细胞生物学]
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参考文献8

  • 1KLEIN G. Cancer, apoptosis, and nonimmune surveillance [J]. Cell Death and Differentiation, 2004,11 : 13-17.
  • 2NOTEBORN M H, de BOER G F, van ROOZELAAR D J, et al. Characterization of cloned chicken anemia virus DNA that contains all elements for the infectious replicationcycle [J]. J Virol, 1991, 65 (6): 3131 -3139.
  • 3ZHUANG S M, SHVARTS A, Van ORMONDT H, et al.Apoptin, a protein derived from chicken anemia virus,induces p53 - independent apoptosis in human osteosarcoma cells[J]. Cancer Res, 1995, 55(3) : 486 -489.
  • 4Notebom MHM, van der Eb A J, Apoptin (R) induces apoptosis in transformed cells speci -fically: Potentials for an anti -tumor therapy [ J ]. Biogenic Amines,1999, 15 (1) :73 -91.
  • 5谭芳,张玉静,徐彦波,刘万臣,张艳宇,柳凤琴.凋亡素(apoptin)诱导人白血病细胞的凋亡[J].中国兽医学报,2001,21(6):571-572. 被引量：4
  • 6PIETERSEN A M, van der Eb M M, RADEMAKER H J. Specific tumor killing with adenovirus vectors containing the apoptin gene[J]. Gene Ther,1999, 6 (5) :882- 892.
  • 7Danen Van Oorschot A A, FISCHER D F, GRIMBER-GEN J M. et al. Apoptin induces apoptosis in human transformed and malignnnant cells but not in normal cells[J]. Proc Natl Acad Sci USA, 1997, 94( 11 ):5843 -5847.
  • 8ZHANG Y H, ABRAHAMS P J, Van drr Eb A J, et al.Response to apoptin of diploid fibroblasts from cancerprone syndromes [ J ]. Dev Biol ( Basel), 2001, 106:359-365.

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哈尔滨工业大学学报
2005年 第8期

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