摘要
目的以无关个体为研究对象,研究鄂温克族人群X染色体5个STR位点的遗传多态性,建立群体遗传数据库。方法用聚合酶链反应(PCR)、变性聚丙烯凝胶电泳和银染的方法,检测98名中国鄂温克族DXS7423,DXS7424,DXS7132,DXS7133,DXS6804位点的重复序列长度的变化。结果5个STR位点在鄂温克族群体均具有遗传多态性,χ2检验表明多态性分布符合Hardy-Weinberg平衡定律。结论中国鄂温克族X染色体5个STR基因座群体遗传数据资料,可用于法庭科学个体识别、亲子鉴定及其他人类学研究。
Objective To study the genetic polymorphism of five STR loci on X-chromosome in 98 Ewenke population of China and establish the related genetic database. Methods The sequence variation of DXS7423,DXS7424,DXS7132,DXS7133,DXS6804 loci on X-chromosome were analyzed by PCR following PAG electrophoresis and silver staining. Results The genetic data of the 5 X-chromosome STRs in Ewenke ethnic group of China is in accordance with Hard-Weinberg equilibrium. Conclusion Five X-STR loci could be used in forensic identification and population genetic research.
出处
《法医学杂志》
CAS
CSCD
2005年第3期192-194,共3页
Journal of Forensic Medicine