摘要
目的探讨人死后肋软骨及牙髓细胞平均DNA含量与死亡时间(PMI)的相关性,寻求推断PMI的新方法。方法应用细胞图像分析技术分析高温(30～35℃)及低温(15～20℃)环境下人死后0～15d两种组织细胞平均DNA含量的降解情况。结果两种组织细胞平均DNA含量随死后时间的延长而逐步降解,其中低温组牙髓细胞平均DNA含量的降解在死后0～4d内有一个降解平台期。统计分析两组温度下两种组织细胞平均DNA含量与PMI有显著的负相关性(P<0.01)。结论依据两种组织细胞平均DNA含量的降解可进行PMI推断。
Objective To probe the correlation between the postmortem interval （PMI） and the DNA degradation of costicartilage and dental pulp cells in human being after death ,and to seek a new method for estimating PMI. Methods The image cytometry was used to measure the DNA degradation under different ambient temperatures （30-35℃,15-20℃） in 0-15 days after death. Results The average DNA content of two kinds of tissue was degradated with the prolongation of PMI. But there was a plateau period of 0-4 days for dental pulp cells of human being in 15-20℃.There was a high negative correlativity P〈0.01 between the average DNA content and PMI. Conclusion PMI could be estimated accurately according to the DNA degradation of costicartilage and dental pulp cells in human being after death.
出处
《法医学杂志》
CAS
CSCD
2005年第3期174-176,共3页
Journal of Forensic Medicine
关键词
死亡时间
牙髓
肋软骨
DNA降解
postmortem interval
dental pulp
costicartilage
DNA degradation
