摘要
目的观察大鼠DAI损伤后β-APP表达和Gless神经纤维轴索染色在诊断DAI损伤及判断损伤时间的价值。方法按Marmarou法复制大鼠DAI损伤模型,脑组织常规取材后进行β-APP及Gless氏神经纤维轴索染色观察。结果β-APP及Gless氏染色法在大鼠DAI损伤后0.5h即可见神经轴索断裂、扭曲变形、增粗膨大,12h以后可见到轴索收缩球。二种方法均显示DAI损伤的病理形态学变化,伤后12h明显,1d达到高峰,3d后开始修复,10d后基本恢复正常。β-APP表达强度在实验组不同时间存在着明显的差异,即3h呈明显阳性表达,1d达到高峰,3d后逐渐减弱,10d基本恢复正常。结论β-APP免疫组织化学染色法及Gless氏神经纤维轴索染色法,对DAI的早期诊断具有重要应用价值,并能从病理形态学上反映DAI损伤的时序性。β-APP表达强度变化是推断早期DAI损伤时间的重要参考指标。
Objective To explore an method for diffuse axonal injury （DAI） diagnosis and injury time estimation, the changes of β-APP immunoreactivity and to observe the morphology of axonal in different parts of brain after experimental DAI injury. Methods The animal models of DAI was established according to the Marmarou＇s method. Immunohistochemistry and Gless staining were performed to observe the changes of β-APP expression and the morphology of axon with the time elapsed after the DAI injury. Results In the brain injury group, the morphologic changes of axon in brain stem were showed as twisted,broken and swellen at 0.5h , and the myelin sheaths broken could be observed, the retraction ball was found at 12 h. Those morphology changes further progressed at 12h, reached to peack up to 1 d, then repaired at 3d, and recovered at 10d; Meanwhile the analysis of β-APP immunoreactivity was also showed a time-dependent difference as fellows： β-APP expression begin at 3 h, increased its immunoreactivity at 12h, reached to maximize at 1 d, decreased after 3 d, returned to basal level at 10d. Conclusions The results suggest that β-APP immunohistochemistry combine with Gless staining be sensitive methods for DAI diagnosis, they could discover the time-dependent changes of the axonal morphology.The changes β-APP are quite regular and could be used for timing DAI injury.
出处
《法医学杂志》
CAS
CSCD
2005年第3期165-168,F0003,共5页
Journal of Forensic Medicine
