摘要
目的:在造成急性视网膜光损伤的光照条件下,通过不同人工晶状体下比较视网膜组织及超微结构的变化,以观察AcrysofNatural对视网膜的保护作用。方法:在无菌条件下将新鲜离体猪眼制成眼杯,利用显微镜光源对视网膜后极部照射,使相应视网膜蓝光光照强度为30J/cm2 或40J/cm2,进行单独光照、透过Acrysof一片IOL、透过Acrysof Natu-ral,照射后培养48h,对相应部位视网膜组织,HE染色光镜、透射电镜下观察。结果:未照射部位:视网膜各层次结构清晰,神经上皮层内各细胞排列规则,整齐,RPE 细胞排列紧密均匀,色素颜色一致;单独光照和透过Acrysof一片式IOL:光感受器层部分细胞丢失,外核层可见固缩核,浓密,内颗粒层细胞大量丢失,内丛状层内空泡明显增多,RPE 表面,细胞大小不均,可见肿胀、缺失,细胞内色素浓密、聚集成块;透过Nat-ural 一片:与未照射处相比,视网膜各层次结构仍有部分破坏,但程度明显减轻。结论:30J/cm2 与40J/cm2 蓝光光照强度均可造成新鲜离体猪视网膜的急性光损伤;Acrysof一片与直接光照相比对视网膜的光损伤形态学没有明显改变;AcrysofNatural与Acrysof一片相比具有减弱光损伤的作用。
AIM： To investigate whether the Acrysof Natural has the protective function for retina from blue light in morphology. METHODS： Fresh porcine eye cups were formed in vitro. Blue light beam between 420-450nm spectrum eradiated the porcine retina and cultured RPE cells in 30.1/cm^2 and 40.1/cm^2, respectively. The adjacent region in 3mm diameter was eradiated in various ways： exposed directly to light, through AcrySof one piece IOL, PMMA IOL, AcrySof Natural IOL, and without light. Then the eye cups were cultured for 48h. Lastly, tissue and cell structure were observed with light microscope and transmission electron microscope （TEM）. RESULTS： In the retinal region without light, the structure of every layer was clear, cells in neuroepithelial layer arrayed in rule, some bubble presented in external granular layer and internal granular layer, RPE cells were compact, and the color of pigment article was coincident. In the region with direct blue light and that with 30J/cm^2＋Acrysof one-piece/ PMMA, cells on photoreceptor and external granular layer were lost partially, bubble increased, RPE cells were with different sizes, and cell edema, cell lost and pigment article cluster could be seen.. In region with 30J/cm^2＋Natural, a little disorgani- zation could be seen comparing to that without light, but more normal than those with Acrysof and direct eradiation. When the power was 40.1/cm^2, the situation was similar to that with 30J/cm^2 but more severe. CONCLUSION： ① the blue light intensity in 30J/cm^2 and 40J/cm^2 could both induce the acute retinal light in- jury, ② AcrySof one piece IOL and other PMMA IOL have no obvious effect no retina comparing to direct eradiation, ③AcrySof Natural can weaken the injury of blue light to some extent.
出处
《国际眼科杂志》
CAS
2005年第4期613-617,共5页
International Eye Science
