摘要
目的:检测骨髓基质细胞系Kusa-A1成骨分化过程中Notch信号相关分子的表达变化,分析Notch信号途径在成骨细胞分化和骨形成中的可能作用.方法:培养小鼠Kusa-A1细胞,在常规培养和诱导培养(添加维生素C和β磷酸甘油)条件下用Real-time PCR方法分别检测Delta1、Jagged1、Notch1、CBF1、HES1基因的表达变化.同时也检测了成骨标志基因骨桥蛋白(OPN)的表达变化.结果:常规培养下Kusa-A1细胞汇片后0～5d,OPN表达有轻微下调,Notch相关分子表达维持不变或有轻微下调.诱导培养条件下细胞汇片后0～5d,OPN表达上调,而Delta1、Jagged1、Notch1、HES1表达大幅度下调,惟有CBF1表达有轻度上调趋势.结论:Notch信号分子表达与Kusa-A1细胞成骨分化负相关,Notch信号对细胞成骨分化可能有抑制作用,而CBF1则可能对成骨细胞分化有正向调节作用.
Objective： To investigate the expression of Notch -related genes in mouse mesenchymal progenitor cell Kusa -A1 and analyze the possible role of Notch pathway in osteogenic differentiation. Methods： Kusa- A1 cells were cultured under normal or mineralization - inducing condition. Total RNA was isolated at day 0, 1, 3, 5 after confluence. Realtime PCR was used to detect the expression of Dehal, Jaggedl, Notchl, CBF1, HES1 genes. Expression of osteopontin （OPN） was also examined. Results： Under normal culture condition, OPN expression was slightly down -regulated and level of Notch - related genes remained unchanged or showed slight down - regulation. After the addition of L - ascorbic acid - 2 - phosphate and 13 - glycerophosphate, known to induce differentiation in osteogenic cells, OPN expression was up - regulated but expression of Dehal, Jaggedl, Notchl and HES1 all showed striking down - regulation. Interestingly, CBF1 expression exhibited slight up -regulation. Conclusion： Notch pathway is involved and may exert a suppression role in the differentiation of osteogenic cells. However, CBF1, transcriptional mediator of Notch signaling, may have a positive effect on osteogenic differentiation.
出处
《口腔医学研究》
CAS
CSCD
2005年第4期389-392,共4页
Journal of Oral Science Research
基金
课题由日中笹川医学奖学金资助
