摘要
目的:建立纯化原代培养的小鼠胚胎腭突间充质细胞的纯化方法。方法:应用D ispase酶4℃消化处理离体小鼠胚胎腭突18 h,通过振荡法使上皮和间充质分离,吸去上皮片。结果:应用流式细胞仪检测,胚胎腭突间充质细胞内几乎不含上皮细胞。结论:本方法为体外研究提供了理想的模型。
Objective： To establish an effective purification method for primary cuhure of mouse embryonic palatal mesenchymal（EPM） cells. Methods： The dissected embryonic palatal shelves were incubated with Dispase at 4℃ for 18 hours, and then the organ rudiments were agitated, a small -bore pipette was used to separate the loosened epithelium from mesenchymal. Results： The EPM cells contained hardly epithelial cells by flow cytometry analysis. Conclusion： This method system provides a valuable model for studies of EPM cells in vitro,
出处
《口腔医学研究》
CAS
CSCD
2005年第4期365-366,共2页
Journal of Oral Science Research
基金
国家自然科学基金(编号:30371552)
关键词
胚胎
腭间充质细胞
细胞培养
纯化
Embryo Palatal mesenchymal cell Cell cuhure Purification
