摘要
目的:研究转录因子Twist、上皮性钙黏附素(E-Cadherin)和波形蛋白(Vimention)在非小细胞肺癌(NSCLC)中的表达,探讨其与非小细胞肺癌浸润转移及预后之间的关系.方法:采用SP免疫组织化学、Western blot和RT-PCR方法检测80例非小细胞肺癌组织Twist、E-Cadherin和vimention蛋白和mRNA,20例癌旁正常组织作对照.另采用免疫细胞化学法检测68例非小细胞肺癌所致胸腔积液中肿瘤细胞的表达.结果:Twist和Vimentin在NSCLC组织及NSCLC胸水标本中的表达显著高于癌旁正常肺组织(P<0.01);E-cadherin在NSCLC组织及NSCLC胸水标本中表达显著低于癌旁正常肺组织(P<0.01);NSCLC组织中Twist及E-cadherin表达均与组织分化,临床分期及淋巴结转移情况密切相关(P<0.01);并且Twist表达与E-cadherin表达降低(P<0.01)和Vimentin的表达上调(P<0.01)之间都存在相关性.结论:Twist蛋白在非小细胞肺癌组织及胸腔转移灶中过度表达,可能通过分别上调和下调Vimentin、E-ead-herin的表达,进而在NSCLC的发生发展及浸润转移过程中发挥作用.
Objective: To investigate the expression of Twist,E-cadherin and Vimention in Nonsmell cell lung cancer(NSCLC) and to analyse their effects On the progression,infiltration and metastasis of NSCLC.Methods:The expression of Twist,E-cadherin and Vimention in formalin-fixed and paraffin-embedded tissues and fresh tissue blocks from 80 patients with NSCLC and 68 cases NSCLC cells from pleural fluid was detected by immunohistochemistry,reverse transcription polymerase chain reaction(RT-PCR) and Western blotting,and 20 specimens of tumor surrounding lung tissue were used as control.Results: The expression of Twist and Vimentin was significantly higher in NSCLC and NSCLC cells from pleural fluid than in tumor surrounding lung tissue(P<0.01),and the expression of E-cadherin was significantly lower in NSCLC and NSCLC cells from pleural fluid than in tumor surrounding lung tissue(P<0.01) The expression of Twist and E-cadherin was closely associated with the pathological parameters,clinical stage and lymph node metastasis(P<0.01).Conclusion:The over-expression of Twist protein might play important role in the infiltration and metastasis of NSCLC by down-regulating the expression of E-eadherin and up-regulating the expression of Vimentin.
出处
《辽宁大学学报（自然科学版）》
CAS
2011年第3期235-240,共6页
Journal of Liaoning University：Natural Sciences Edition
