摘要
目的研究反复腹痛患儿及家庭成员幽门螺杆菌(helicobacter pylori,Hp)感染的情况和外周血淋巴细胞和单核细胞人类白细胞抗原(HLA)-DR的表达水平,探讨患儿及家庭成员外周血淋巴细胞和单核细胞HLA-DR表达与Hp感染后反复腹痛的关系.方法应用免疫印迹法和流式细胞术检测13个家庭57名成员及37名志愿者血Hp抗体和外周血血单核细胞和淋巴细胞HLA-DR表达,根据临床症状分为反复腹痛患儿组、反复腹痛家庭成员组及无腹痛对照组,分析和比较3组患者的血Hp抗体及亚型和外周血血单核细胞和淋巴细胞HLA-DR表达.结果所有受检者共94人,Hp阳性者共63人,阳性率为67.0%,Hp阳性的63人中,HpⅠ型占80.95%,有腹痛的38人;HpⅡ型占19.05%.在反复腹痛患儿的57名家庭成员中,Hp阳性者54例,阳性率为94.7%.HpⅠ型的占77.8%,Ⅱ型的占22.2%人.家庭内感染Hp菌株相似,存在家庭聚集和链性现象.血单核细胞HLA-DR表达患儿组、腹痛家庭成员组的表达均高于无腹痛组,有统计学差异(P<0.05),而血淋巴细胞HLA-DR表达在三组间差异无统计学意义(P>0.05).结论反复腹痛患儿及其家庭成员中Hp感染率高,有家庭聚集和链性感染发病现象,家庭内感染Hp菌株相似.
Objective To investigate the prevalence and the phenotype of Helicobacter pylori(Hp) infection and the human leukocyte antigen(HLA)-DR expression in the peripheral blood lymphocytes and monocytes in family members of children with recurrent abdominal pain,and to explore the association between HLA-DR expression and Hp-associated recurrent abdominal pain.Methods Venous blood specimen was obtained from 57 family members of 13 children with recurrent abdominal pain and 37 adult volunteers for HLA-DR expression on monocytes by flow cytometry and for the Hp type by Hp immunophenotyping method.Individuals were divided into children with recurrent abdominal pain,adult family members with recurrent abdominal pain and the normal control group according to symptoms.The phenotype of Hp infection and the human leukocyte antigen(HLA)-DR expression in the peripheral blood lymphocytes and monocytes were analyzed and compared among the three groups.Results The positive rate of Hp immunophenotyping antibody test in 94 individuals was 67.0%,and the percent of HpⅠtype was 80.95% while HpⅡtype was 19.05%.The positive rate of Hp in 57 family members of children with recurrent abdominal pain was 94.7%,and the percent of HpⅠtype was 77.8% while HpⅡtype was 22.2%.Hp immunophenotype was similar in the same family.Hp infection had the family cluster and line phenomenon.The expression of HLA-DR in peripheral blood monocytes among three groups had difference(H=10.624,P<0.05),children and family members with recurrent abdominal pain expressed higher than those without recurrent abdominal pain,but the result had no difference in lymphocytes(H=0.966,P>0.617).Conclusions The seropositive rate of Hp antibodies is high in the family members with recurrent abdominal pain.There is the familial cluster and line phenomenon in the family members with recurrent abdominal pain by Hp infection.The higher expression of HLA-DR on monocytes in familial members with recurrent abdominal pain caused by Hp infection may be associated with active immunological function stimulated by Hp chronic infection.
出处
《昆明医学院学报》
2011年第12期77-81,共5页
Journal of Kunming Medical College
基金
云南省自然科学基金资助项目(2009CD163)
