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肺炎衣原体包涵体膜蛋白Cpn0147抗原性分析 被引量：2

Antigenicity Analysis of Inclusion Body Membrane Protein Cpn0147 of Chlamydia Pneumoniae
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摘要 目的:在大肠杆菌中表达纯化融合蛋白GST-Cpn0147,研究其免疫原性。方法:将Cpn0147基因克隆到原核表达载体PGEX-6P2,转化入大肠杆菌XL-Blue,经IPTG诱导表达并纯化;以纯化的融合蛋白免疫BALB/c小鼠,制备免疫血清,ELISA法证实其免疫原性并检测免疫血清的效价,Western-blot检测免疫反应性。结果:在大肠杆菌中表达了GST-Cpn0147融合蛋白,以该蛋白免疫小鼠获得了免疫血清。结论:获得了GST-Cpn0147融合蛋白,并证实其具有免疫原性和免疫反应性。 Objective To express and purify recombinant protein GST-Cpn0147 and to explore its antigenicity.Methods The gene Cpn0147 was cloned into prokaryotic expression vector PGEX-6P2.PGEX-6P2-Cpn0147 was introduced into E.coil XL-Blue and fusion protein GST-Cpn0147 was expressed and purified.Then the purified fusion protein was used to vaccinate Balb/c mice,protein Cpn0147 immunogenicity was detected by ELISA and its immunity responsibility was detected by Western-blot.Results The fusion protein GST-Cpn0147 was successfully expressed in E.coil,serum antibody was obtained from vaccinated mice.Conclusion The fusion protein GST-Cpn0147 was obtained and its immunogenicity and immunoreactivity were proved.
作者 童伟 李峥 贾天军
机构地区 湖北医药学院附属襄阳医院检验科 襄阳市中心医院消化内科 河北北方学院医学检验学院
出处 《湖北医药学院学报》 CAS 2011年第6期569-572,共4页 Journal of Hubei University of Medicine
关键词 包涵体膜蛋白 抗原性 原核表达 免疫原性 Inclusion body membrane protein Antigenicity Prokaryotic expression Immunogenicity
分类号 R374 [医药卫生—病原生物学]
  • 相关文献

参考文献12

  • 1童伟,张战军,贾天军.肺炎衣原体Cpn0147基因的克隆、表达与定位及其重组蛋白的生物信息学分析[J].中国病原生物学杂志,2009,4(8):567-569. 被引量：3
  • 2Rockey DD,,Scidmore MA,Bannantine JP,et al.Pro-teins in chlamydial inclusion membraneMicrobes and Infection,2002.
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二级参考文献5

  • 1Kuo CC,Jackson LA,Campbell LA,et al. Chlamydia pneumoniae (TWAR) [J]. Clin Microbiol Rev,1995,8(4) :451--461.
  • 2Kaperonis EA, Liapis CD, Kakisis JD, et al. Inflammation and Chlamydia pneumoniae infection correlate with the severity of peripheral arterial disease[J]. Eur J Vase Endovasc Surg, 2006,31 (5):509--515.
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共引文献2

同被引文献18

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引证文献2

湖北医药学院学报
2011年 第6期

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