摘要
目的:研究丝裂霉素C-聚氰基丙烯酸正丁酯磁性纳米球(MMC-PBCA-MNPS)胶体溶液对裸鼠人肝癌模型的靶向治疗效果.方法:建立了30只裸鼠人肝癌模型的裸鼠,以给药不同被随机分为6组(n=5),各组分别在尾iv生理盐水(对照组),MMC生理盐水和MMC-PBCA-MNPS胶体溶液,给予MMC-PBCA-MNPS胶体溶液又被分为施加磁场组和不施加磁场组.治疗结束后摘取肿瘤分别称质量,计算抑瘤率.结果:在肿瘤部位施加4950Gs的外磁场,iv给药5次即有明显的抑制肿瘤生长作用,其瘤质量抑制率为73.9%(低剂量)、84.2%(中剂量)、88.6%(高剂量),在肿瘤部位不施加磁场的瘤重抑制率为54.0%(中剂量),高于MMC生理盐水组(27.6%,P<0.05).病理结果也证明了上述结果.结论:MMC-PBCA-MNPS)胶体溶液结合体外磁场可显著提高MMC的抗瘤效果,且毒副作用明显减低.
AIM： To study the targeting therapeutic effect of mitomycin C polybutylcyanoacrylate magnetic nanoparticles （MMC-PBCA-MNPS） on nude mice transplanted subcutaneously by Bel-7402 cell lines. METHODS： Thirty nude mice transplanted subcutaneously by Bel-7402 cell lines were randomly divided into six groups （ n = 5）. Each group was given, through tail vein, mitomycin normal saline （MMC）, MMC-PBCA-MNPS and saline as control respectively. The groups of MMC-PBCA-MNPS were divided into magnetic field and nonmagnetic field. Tumors were moved and weighted to calculate the ratio of tumor inhibition at the end of the treatment. RESULTS： Under the influence of an external magnetic field of 4950Gs close to tumors in vitro, the inhibition rate of tumor weight growth by MMC-PBCA- MNPS was 73.9% （low dosage）, 84.2% （ moderate dosage） and 88.6% （high dosage）, respectively. The inhibition rate of tumor growth by MMC-PBCA-MNPS was .54.0% （moderate dosage） in nonmagnetic field, higher than that of MMC normal saline group（27.6%, P〈0.05）. The pathological examination confirmed the above-mentioned resuits. CONCLUSION： MMC-PBCA-MNPS with an external magnetic field significantly increases the anti-tumor effects of MMC and decreases its side effects and cytotoxicity.
出处
《第四军医大学学报》
北大核心
2005年第16期1510-1512,共3页
Journal of the Fourth Military Medical University
