摘要
目的:探讨TMD患者关节液中MMP-2的表达水平与关节病损程度的关系.方法:用ELISA法检测TMD患者的126侧关节和健康志愿者的32侧关节的关节液标本中的MMP-2水平.患者关节液标本分为(1)C/S组;(2)ID组;(3)OA组;健康志愿者关节液为对照组.结果:关节液中MMP-2含量:OA组(43±14)μg/L,ID组(35±13)μg/L,C/S组(30±8)μg/L,对照组(21±5)μg/L.统计学检验:OA组与ID组对照组比较均有差异(P<0.0083);C/S组与对照组间无差异.OA组与C/S以及ID组与C/S组间比较亦无差异.结论:MMP-2参与了TMJ关节软骨的分解;TMD患者关节液中MMP-2的表达水平随着关节不同的病变增加而改变,表明它们不仅参与了关节组织的破坏性病理改变,而且与关节疾病程度密切相关,其含量可以作为评估TMJ软骨是否发生病理损害的客观生化指标.
AIM： To study the expression of MMP-2 in synovial fluid in temporomandibular joints （TMJ） from patients with temporomandibular disorder （TMD）. METHODS： Samples from 126 TMJs in TMD patients were divided into three groups according to clinical examination and imaging findings： capsulitis and synovitis （C/S） group （ n =24）, internal derangement （ID） group （ n = 58） and osteoarthritis （OA） group （n =44）. Samples from asymptomatic volunteers served as control group （ n = 32）. The concentration of MMP-2 in TMJ synovial fluid was measured using enzyme-linked immunosorbent assay. RESULTS： The levels of MMP-2 in OA group （43 ± 14） μg/L and ID group （35 ± 13） μg/L were significantly higher than that of control group [ （21±5） μg/L] （P〈0.0083）. But there was no difference between C/S group （30 ＋ 8） μg/L and control group. No difference was found between C/S group and ID and OA groups. CONCLUSION： MMP-2 participates in the pathological destructive process of TMJ cartilage. The MMP-2 level in synovial fluid of patients with OA and ID is significantly higher than that of healthy volunteers. This indicates that MMP-2 is not only involved in the pathological destructive process of TMJ cartilage, but also is positively correlated with the lesion degree of TMJ, suggesting that MMP-2 can be used as a biochemical marker for the lesion degree of TMJ.
出处
《第四军医大学学报》
北大核心
2005年第16期1478-1480,共3页
Journal of the Fourth Military Medical University
基金
北京军区"十五"医学科学技术研究资助项目(02B011)
关键词
颞下颌关节功能紊乱综合征
颞下颌关节
滑液
明胶酶A
酶联免疫吸附试验
temporomandibular joint dysfunction syndrome
temporomandibular joint
synovialfluid
grelatinase A
enzyme-linked immunosorbent assay
