期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

牙本质涎磷蛋白G0代转基因小鼠的获得 被引量：2

Generation of transgenic mouse founders of DSPP
暂未订购
导出
摘要 目的:构建四环素调控性牙本质涎磷蛋白(DSPP)转基因体系中的受控小鼠系.方法:从质粒pBluescript-DSPP中,将小鼠DSPP编码序列亚克隆到pTRE2的NotI/SalI位点间,构建转基因载体,经酶切鉴定和测序确认后,命名为pTRE2-DSPP;将XhoI酶切线性化的载体DNA纯化后,显微注射到小鼠受精卵的雄原核中,挑选生长状态好的受精卵,移植到假孕母鼠的输卵管.仔鼠出生3wk后,用PCR及SouthernBlot检测阳性小鼠.结果:注射的受精卵共存活657枚,移卵至28只假孕母鼠后,产仔85只,PCR检测10只为阳性,SouthernBlot检测6只为阳性.SouthernBlot检测阳性小鼠分别传代,开始建系.结论:显微注射方法获得了DSPP转基因受控小鼠的G0代. AIM： To establish tetracycline-responsive regulated transgenic mice of dentin sialophosphoprotein （DSPP）. METHODS： DSPP coding sequence was contained in plasmid pBluescdpt-DSPP and the transgenic vector was constructed by subcloning it into pTRE2 by Notl/Sail digestion. Following identification by enzyme digestion and sequencing, the final plasmid was named pTRE2DSPP, After linearized by Xhol and recovery, the plasmid was microinjected into the male pronucleus of the zygotes and the zygotes in good condition were implanted into pseudopregnant mice. The tail DNA of 3 week-pups was tested by PCR and Southern blot. RESULTS： Six hundred and fifty-seven embryos were implanted to 28 recipient pseudo- pregnant mice, with 6 of the 85 pups carrying the transgene. The establishment of the transgenic line progressed from the 6 positive ones. CONCLUSION： The founders of the tetracycline-responsive regulated transgenic mice of DSPP are established successfully.
作者 孙汉堂 肖明振 吴补领 徐国江 费俭
机构地区 第四军医大学口腔医学院口腔内科 上海南方模式生物研究中心 中国科学院上海生物化学与细胞生物学研究所
出处 《第四军医大学学报》 北大核心 2005年第16期1454-1456,共3页 Journal of the Fourth Military Medical University
关键词 牙 牙本质涎磷蛋白 小鼠转基因 微量注射 tooth dentin sialophosphoprotein mice,transgenic microinjections
分类号 R780.2 [医药卫生—口腔医学]
  • 相关文献

参考文献4

二级参考文献17

  • 1[1]Harada H, Kettunen P, Jung HS, Mustonen T, Wang YA, Thesleff I. Localization of putative stem cells in dental epithelium and their association with Notch and bFGF signaling [J]. J Cell Biol, 1999; 147(1):105-120.
  • 2[2]Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo [J]. Proc Natl Acad Sci USA, 2000; 97 (25): 13625-13630.
  • 3[3]Jernvall J, Thesleff I. Reiterative signaling and patterning during mammalian tooth morphogenesis[J]. Mech Dev, 2000; 92(1):19-29.
  • 4[4]Tucker AS, Sharpe P. Molecular genetics of tooth morphog enesisand patterning: the right shape in the right place [J]. J Dent Res, 1999; 78(4):826-834.
  • 5[5]Tucker AS, Matthews KL, Sharpe P. Transformation of tooth type induced by inhibition of BMP signaling [J]. Science, 1998;282(5391):1136-1138.
  • 6[6]Peters H, Neubuser A, Balling R. Pax genes and organogenesis: Pax9 meets tooth development [J]. Eur J Oral Sci, 1998;106(Suppl 1): 38-43.
  • 7[7]Weiss K, Stock D, Zhao Z. Dynamic interactions and the evolutionary genetics of dental patterning [J]. Crit Rev Oral Biol Med,1998; 9(4):369-398.
  • 8[8]Johansson CB, Momma S, Clarke DL, Risling M, Lendahl U, Frisen J. Identification of a neural stem cell in the adult mammalian central nervous system [J]. Cell, 1999; 96(1):25-34.
  • 9[9]Sanz E, Alvarez-Mon M, Martinez-A C, De La Hera A. Human cord blood CD34+Pax-5+ B-cell progenitors: Single-cell analyses of their gene expression profiles [J]. Blood, 2003; 101(9):3424-3430.
  • 10[10]Young CS, Terada S, Vacanti JP, Honda M, Bartlett JD, Yelick PC. Tissue engineering of complex tooth structures on biodegradable polymer scaffolds [J]. J Dent Res, 2002; 81(10):695-700.

共引文献24

同被引文献11

  • 1Volpers C,Kochanek S.Adenoviral Vectors for gene transfer and therapy.J Gene Med,2004,6 (Suppl 1):164-171.
  • 2Thomas D,Suthanthiran M.Optimal modes and targets of gene therapy in transplantation.Immunol Rev,2003,196(1):161-175.
  • 3Kawabata K,Sakurai F,Koizumi N,et al.Adenovirus vector-mediated gene transfer into stem cells.Mol Pharm,2006,3(2):95-103.
  • 4MacDougall M,Simmons D,Luan X,et al.Dentin phosphoprotein and dentin sialoprotein are cleavage products expressed from a single transcript coded by a gene on human chromosome 4.Dentin phosphoprotein DNA sequence determination.J Biol Chem,1997,272(2):835-842.
  • 5Yamakoshi Y,Hu JC,Fukae M,et al.Dentin glycoprotein:the protein in the middle of the dentin sialophosphoprotein chimera.J Biol Chem,2005,280(17):17472-17479.
  • 6Bègue-Kim C,Krebshach PH,Bartlett JD,et al.Dentin sialoprotein,dentin phosphoprotein,enamelysin and ameloblastin:tooth-specific molecules that are distinctively expressed during murine dental differentiation.Eur J Oral Sci,1998,106(5):963-970.
  • 7Narayanan K,Srinivas R,Ramachandran A,et al.Differentiation of embryonic mesenchymal cells to odontoblast-like cells by overexpression of dentin matrix protein 1.Proc Natl Acad Sci USA,2001,98(8):4516-4521.
  • 8He TC,Zhou SB,Da Costa LT,et al.A simplified system for generating recombinant adenovirus.Proc Natl Acad Sci USA,1998,95(5):2509-2514.
  • 9Gordon JW,Scangos GA,Plotkin DJ,et al.Genetic transformation of mouse embryos by microinjection of purified DNA[J].Proc Natl Acad Sci USA,1980,77:7380.
  • 10焦成松,周永兴,朱德生,贾战生,冯志华.乙型肝炎病毒S基因经显微注射导入SMMC-7721细胞核内的表达[J].世界华人消化杂志,2000,8(1):25-27. 被引量：8

引证文献2

二级引证文献3

第四军医大学学报
2005年 第16期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部