摘要
目的探讨8-氯腺苷是否会影响肿瘤细胞的表皮生长因子受体(EGFR)及其相关信号蛋白的表达.方法人胃癌BGC-823细胞与不同浓度8-氯腺苷共孵育.SRB染色法观察细胞增殖;Western蛋白印迹法观察细胞增殖相关信号蛋白的表达.结果8-氯腺苷1.0, 2.5,5.0,7.5和10 μmol*L-1作用24 h,浓度依赖性抑制BGC-823细胞增殖,抑制率分别为19.7%,31.8%, 40.1%, 49.8%和56.7%.7.5 μmol*L-18-氯腺苷作用BGC-823细胞24, 48和72 h,抑制率分别为49.8%, 49.1%和45.6%.Western蛋白印迹结果表明,8-氯腺苷2.5～7.5 μmol*L-1作用24 h,BGC-823细胞EGFR蛋白表达减少;Raf-1蛋白表达变化不显著;p-MEK和p-ERK磷酸化蛋白表达增加.结论8-氯腺苷可抑制BGC-823细胞增殖;改变EGFR-Raf/MEK/ERK等一系列信号蛋白的表达可能是其抑制细胞增殖的重要途径之一.
AIM To study whether 8-chloroadenosine can influence the expression of epidermal growth factor receptor （EGFR） and related signal proteins. METHODS In vitro. BGC-823 cells were incnbated with different concentrations of 8-chloroadenosine, The grouth inhibition was evaluated with sufforhodamine B assays. Western-blot analysis was used to determine expression of signal transduction related proteins in BGC-823 cells. RESULTS After 24 h incubation, 8-chloroadenosine 1.0, 2.5, 5.0, 7.5 and 10μmol·L^-1 ion-dependently inhibited the growth of BGC-823 cells by 19.7%, 31.8%, 40. 1%, 49.8% and 56 7%. When incubated with 8-chloroadenosine 7.5 μmol·L^-1 for 24, 48 and 72 h. growth of BGC-823 cells wasinhibited bv 49.8%, 49. 1% and 45.6%, respectively. 8-Chloroadenosine 2.5 - 7.5μmol·L^-1, 24 h, caused a eoneentration-dependent decrement on EGFR, and increment on p-MEK and p-ERK protein expression. The decrement of Raf-1 protein expression was not very remarkable, CONCLUSION 8-Chloroadenosine inhibits proliferation of cancer cells, which may be related to changing EGFR-Raf/MEK/ERK cascade.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2005年第4期280-283,共4页
Chinese Journal of Pharmacology and Toxicology
