摘要
浅麻醉Ｗｉｓｔａｒ大鼠４９只，用辐射热照尾部作为伤害性刺激，以痛兴奋神经元（ｐａｉｎｅｘｃｉｔａｔｉｏｎｎｅｕｒｏｎｓＰＥＮ）诱发放电频率减少、痛抑制神经元（ｐａｉｎｉｎｈｉｂｉｔｉｏｎｎｅｕｒｏｎｓＰＩＮ）诱发放电频率增加和甩尾反射潜伏期（ｔａｉｌｆｌｉｃｋｌａｔｅｎｃｙＴＦＬ）延长为镇痛效应，观察脑室注射不同剂量吗啡对尾核中ＰＥＮ、ＰＩＮ及ＴＦＬ的同时影响。表明，脑室注射５０μｇ／１０μｌ吗啡，尾核中ＰＥＮ、ＰＩＮ放电呈镇痛效应（Ｐ＜０．０５），ＴＦＬ影响不显著（Ｐ＞０．０５）。注射７０、１００μｇ／１０μｌ吗啡，尾核中ＰＥＮ、ＰＩＮ放电和ＴＦＬ均出现显著镇痛作用，且呈量效关系；吗啡对ＴＦＬ的作用高峰晚于对尾核中ＰＥＮ、ＰＩＮ放电的作用，对ＴＦＬ影响时间长。说明，吗啡对大鼠尾核中ＰＥＮ、ＰＩＮ两者的影响先于其对甩尾反射抑制效应的出现。
The effects of different doses of morphine administered to the lateral ventricle on firing of pain excitation neurons(PEN)and pain inhibition neurons(PIN) and tail flick latency(TFL) were observed simultaneously on forty nine lightly anesthetized Wistar rats with the radiant heat as noxious stimulation.The decrease of firing rate of PEN and the increase of firing rate of PIN and the Prolongation of TFL were regarded as antinociceptive effect.The results showed that the TFL did not change significantly while antinociceptive effect already appeared in PEN and PIN,when lower dosage(50μg/10μl) of morphine was used. However, when the dosage of morphine increased to 70, 100μg/10μl, the TFL increased significantly as well as the change in PEN and PIN, although the peak of the change in TFL appeared later and the effect on TFL lasted longer.The results suggest that the influence of morphine could be detected in both PEN and PIN in the caudate nucleus before its behavioral effect emerges.
出处
《神经科学》
SCIE
CAS
1995年第3期98-102,共5页
Chinese Journal of Neuroscience
关键词
疼痛
兴奋神经元
抑制神经元
甩尾
反射
吗啡
pain excitation neuron
pain inhibition neuron
tail flick latency
morphine
caudate nucleus
lateral ventricle
