摘要
目的 研究凋亡抑制基因Livin在原发性肝癌中的表达,并探讨其与Caspase-3表达的相关性.方法 采用逆转录多聚酶链式反应(RT-PCR)方法检测Livin和Caspase-3在46例原发性肝癌,28例癌旁组织,12例正常肝组织中的表达,分析二者之间相关性.结果 Livin基因在肝癌组织、癌旁组织、正常肝组织表达依次降低,其表达与原发性肝癌患者的年龄、性别、肿瘤大小、是否合并乙肝肝硬化、分化程度、甲胎蛋白等差异均无统计学意义(P＞0. 05),而与肝癌是否发生转移有关(P<0. 05);Caspase-3在上述组织中依次升高,差异均有统计学意义,二者在肝癌组织中表达负相关(rs=-0. 333, P<0.05).结论 Livin在肝癌组织中高表达,可能和Caspase-3在原发性肝癌细胞凋亡中起重要作用,可能是原发性肝癌生物学行为和预后重要标记物之一.
Objective To explore the expression of Livin in hepatocellular carcinoma and its relationship with Caspase-3. Methods Expression of Livin and Caspase-3 were detected in hepatocellular carcinoma(n=46), para-carcinoma tissues(n=28) and normal liver tissues(n=12) by reverse transcription polymerase chain reaction (RT-PCR). Results The expression of Livin was found lowered in order in hepatocellular carcinoma, para-carcinoma tissue and normal liver tissue, which had no relationship with age, gender, the sizes of HCC, pathological types and AFP,etc (P＞0.05). But it had relationship with the metastasis of HCC(P<0.05).The expression of Caspase-3 was found increased in order in those tissue. Livin expression was negatively related to Caspase-3 expression in hepatocellular carcinoma(rs=-0. 333, P<0.05).Conclusions There is high positive expression rate of Livin in HCC,which may had an important role combined with Caspase-3 in apoptosis of HCC and be a valuable diagnostic and prognostic marker of HCC.
出处
《中国实用医刊》
2010年第8期13-14,共2页
Chinese Journal of Practical Medicine
