摘要
目的 探讨缺氧缺血性脑损伤(HIBD)时血清神经元特异性烯醇化酶(NSE)的表达规律以及黄芪的干预作用.方法取孕中晚期新西兰兔36只,随机分为对照组、缺氧组及黄芪干预组.于各组新生兔出生后1、2、3、7 d采血,用酶联免疫吸附法(ELISA)测定血清NSE含量.结果对照组各时间点血清NSE含量比较差异无统计学意义(P均>0.05);缺氧组、黄芪干预组NSE水平较对照组明显升高,但黄芪干预组明显低于缺氧组,差异有统计学意义(P均<0.01).动态监测血清NSE发现,缺氧组、黄芪干预组血清NSE高峰出现在兔出生后1 d,并随日龄增长含量逐渐下降,7 d时仍未降至正常水平,且两两比较差异有统计学意义(P均<0.05).结论血清NSE变化与HIBD程度密切相关,HIBD程度越重,NSE升高越明显.黄芪干预后可降低缺氧动物血中NSE水平,推断黄芪对缺氧时的脑组织有保护作用.
Objective To investigate the expression law of neuron-specific enolase(NSE) in serum of newborn rabbits with hypoxia／ischemia brain damage(HIBD)and protection of astragalus (黄芪) ．Methods Thirty—six New Zealand rabbits in the medium—and terminal pregnancy-term were taken,and then they were randomly divided into three groups lcontrol，oxygen deprivation and astragalus intervention groups ．After they were born。blood was collected from newborn rabbits at 1，2，3 and 7 days respectively．Enzyme linked immunosorbent assay(ELISA)was used to quantitatively estimate the content of NSE in the serum．Results No significant statistical significance was obtained indifference of NSE content in serum in the control group at the different time points (all P>0．05) ．The NSE levels in the oxygen deprivation group and the astragalus intervention group were significantly increased compared with those in the control group， but NSE level in the astragalus intervention group was obviously lower than that in the oxygen deprivation group(all P<O.01)．With dynamic detection of NSE in serum，the NSE content in the oxygen deprivation group and the astragalus intervention group reached a peak 1 day after birth．The content of NSE in serum gradually decreased with time，and not dropped to normal level after 7 days ．There was statistical significance in difference of NSE content with paired—comparisons( all P<O．05)．Conclusion The result indicates that the change of NSE in serum is closely related with the HIBD level ．The more severe is the HIBD level，the more significant elevation is the NSE level.Astragalus intervention can decrease NSE level in the serum of hypoxic animal,that indicates the astragalus may play a role in protection of brain tissue from oxygen deprivation.
出处
《中国中西医结合急救杂志》
CAS
北大核心
2009年第6期342-344,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
福建省教育厅科技项目，福建省泉州市科技计划项目
