摘要
目的:探讨亚铁嗪法测定血浆铁假性升高的原因及解决办法。方法:用试管法实验观察反应体系的变化;用血清和血浆两种校准品分别校准生化分析仪,配对测定20例血清和血浆标本。结果:血浆中的纤维蛋白原在测定条件下变性使反应体系产生混浊,从而干扰测定,使结果假性升高,血浆标本结果(169.29±43.80 μ g/dL)与血清标本结果(98.34±47.93 μ g/dL)比较,差异有统计学意义(t=16.14,P<0.001)。血浆定标后测定血浆标本,结果(107.1±37.88 μ g/dL)与对应的血清铁(98.34±47.93 μ g/dL)比较,差异无统计学意义(t=1.994,P>0.05 )。结论:用血浆定标法测定血浆铁可基本消除纤维蛋白原的干扰。
Objective:To discuss the reason of plasma iron false increasing tested with Ferrozine method.Meth- ods:The phenomena of the reactions between samples and reagents were observe in cuvettes.The serum iron and plasma iron were detected in 20 samples in pairs by using the serum and plasma calibrator to calibrate the analyzer, respectively.Results:The precipitate was produced in plasma samples when the reagent 1 added into the cuvettes. With serum calibrator to calibrate the analyzer,the results of iron were significantly higher in plasma samples than that in serum samples(169.29±43.80μg/dL vs 98.34±47.93μg/dL,t=16.14,P<0.001);and with the plasma calibrator to calibrate the analyzer,the results were semblable between plasma samples and serum samples which calibrated with serum calibrator(107.1±37.88μg/dL vs 98.34±47.93μg/dL,t=1.994,P>0.05).Conclusion:The interference caused by fibrinogen denaturation in plasma would be eliminated satisfactorily by using a plasma calibrator.
出处
《现代仪器》
2009年第6期40-41,45,共3页
Modern Instruments
