摘要
烧伤后血清抑制因子的存在业已证实,而且被认为是导致机体防御功能受抑的原因之一。本文的目的是利用在凝胶中免疫沉淀的方法,从正常血清和烧伤血清的对比中探索伤后血清蛋白的异常。从SOS—聚丙烯酰胺凝胶电泳、交叉免疫电泳、Tandem交叉免疫电泳和融合火箭电泳的结果可以看到,正常与烧伤血清之间存在有不同的沉淀区带或沉淀峰。在烧伤血清中存在有分子量较低的异常组分。
Serum suppressive factors were proved to be present and considered to be one of the causes to bring about the suppression of body defence following thermal injury. As a preliminary step to ascertain the presence of immunosuppressive substances, we tried to find out any abnormality in the postburn serum proteins by comparison of burned and normal sera by means of immuno-precipitation-in-gel. Results of SDS-polyacrylamide gel electrophoresis, crossed immunoelectrophoresis, tandem crossed immunoelectrophoresis, and fused rocket immunoelectrophoresis showed that differences of precipitation bands or peaks existed between burned and normal sera, and abnormal constituents with relatively low molecular weight were present in the burned serum.
