摘要
目的:研究吗啡对活性氧自由基的影响.方法:用化学发光法检测.(a)人血嗜中性白细胞受佛波醇刺激产生呼吸爆发的活性氧.(b)黄嘌呤-黄嘌呤氧化酶体系产生的O^(?)_2;(c)AA-Cu^(2+)-酵母多糖产生的·OH;(d)过氧化氢的释放反应.结果:吗啡清除(a),(b),(c),(d)所产生的氧自由基,其IC_(50)值(nmol L^(-1))分别为21.1(13.0—34.0),54.1(50.0—58.5),224.0(128.2—390.8),和66.9(62.9—71.0).结论:吗啡的氧自由基清除作用与吗啡的免疫抑制作用有关.
AIM:To study the effects of morphine on active oxygen free radicals. METHOD:Chemi-luminescence method was used to measure (a) active oxygen generation induced by respiratory burst of neutrophils from human blood stimulated with phorbol myristate acetate (PMA), (b) Superoxide anion (O2) induced by xanthine-xanthine oxidase system, (c) hy-droxyl radical (.OH) generated by ascorbic acid (AA)-Cu2+-zymosan, and (d) the release of H2O2. RESULTS:The (a) , (b) , (c) , and (d) were scavenged by morphine and their median inhibitory concentrations (IC50 and 95 % confidence limits) were 21.1 (13.0-34.0), 54.1 (50.0-58.5), 224.0 (128.2-390.8), and 66.9 (62.9 -71.0), nmol L-1, respectively. CONCLUSION: The immuno-suppressant effect of morphine was related to its free radicals scavenging action.
出处
《中国药理学报》
CSCD
1995年第5期445-448,共4页
Acta Pharmacologica Sinica
