摘要
目的:探讨五味子酚(Sal)对大鼠嗜中性白细胞(Neu)功能的调节作用.结果:Sal 1,10,100μmol·L^(-1)剂量依赖性抑制Neu功能,Sal 100μmol·L^(-1)使Neu表面伪足和皱褶消失,并可降低细胞内钙离子浓度、升高细胞内cAMP水平.结论:Sal可通过影响细胞内钙离子浓度、cAMP水平及细胞表面形态抑制Neu的功能.
AIM: To study the regulatory effect of Schisanhenol (Sal) on function of rat neutrophils. RESULTS: It was found that Sal (1, 10, and 100μmol·L-1) inhibited neu-trophil activities such as chemotaxis, phagocytosis , and superoxide anion production in vitro in a concentration-dependent manner. Changes of surface morphology of neutrophils were observed by scanning electron microscopy, showing that the ruffles and pseu-dopods on neutrophil surface increased under the stimulation by chemotactic peptide N-formyl-Met-Leu-Phe (FMLP). When pre-treated with Sal 100μmol·L-1, the ruffles and pseudopods disappeared and the surface became smooth. Sal 100μmol·L-1 decreased the cytosolic calcium concentration of neutrophilsand increased the intracellular cAMP level. CONCLUSION: These data suggested that Sal could inhibit the function of rat neutrophils through affecting the cytosolic free calcium and cAMP level besides its anti-oxidant activity.
出处
《中国药理学报》
CSCD
1995年第3期234-238,共5页
Acta Pharmacologica Sinica
基金
Project supported by the National Natural Science Foundation of China (№39170232)
HUO Ying-Dong Education Foundation.
关键词
五味子酚
嗜中性粒细胞
白细胞趋化性
钙
schisanhenol
neutrophils
chemotaxis
phagocytosis
calcium
cyclic AMP
cyclic GMP
