摘要
研究了薄层扫描法及薄层－紫外分光光度法测定长春花中长春碱含量的两种方法，在硅胶Ｆ２５４－ＣＭＣ－Ｎａ碱性板上（ＮａＯＨ浓度为０．１ｍｏｌ／Ｌ），以氯仿－甲醇－石油醚（９：１：５）为展开剂，分离了长春花提取液中长春碱和长春新碱等１４种成分。前法采用双波长簿层扫描，λｓ＝２８０ｎｍ，λＲ＝３５０ｎｍ，线性范围在２～１０μｇ．后法采用薄层分离后，样品带用甲醇洗脱后，紫外分光光度法测定含量。后法在５～２５μｇ／ｍｌ间符合比尔定律。两法回收率均在９９％以上，ＲＳＤ在２％以下，两法测定结果基本一致。前法较快速、灵敏、简便。后法不需特殊仪器设备。
This paper deals with two analytical metheds of vinblastine in Catharanthus roseus(L.)G.Don which are TLC scanning and TLC-UV.Fourteen components which were vinblastine, vincristine and so on in extract of Catharanthus roseus (L.)G.Don were separated on ailica gel F254-CMC plates with chloroform-methanol-petroleum ether(9:1:5)as mobile phase.Vinbtastine was determined by using dual wavelength TLC scanner at λs=280 nm,λR=350 nm.The calibration curve was linear in the range from 2 μg to 10 μg for the firot method and 5 μg/ml to 25 pg/ml for the second.The recoveries of both methods were bver 99% and their relative standard deviation was befow 2%.The former was fast, sensitive and simple.The latter didn't need special instruments and its precision wes good too.The extracting methed of vinblastine and the conditions for TLC developins were investigated.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1995年第3期157-159,共3页
Journal of China Pharmaceutical University
关键词
长春花
长春碱
薄层扫描
Catharanthus roseus(L.)G.Don
Vinblastine
TLC Scanning
TLC-UV
