摘要
血浆样品用二氯甲烷提取，采用ＯＤＳ柱，以甲醇－磷酸盐缓冲液－三乙胺（３０：６９．８：０．２）为流动相，炎痛喜康为内标，２５４ｎｍ为检测波长，ＲＰ－ＨＰＬＣ法测定兔口服替诺昔康片后的体内血药浓度。线性范围０．１～３０μｇ／ｍｌ，最低检测用０．１μｇ／ｍｌ（Ｓ／Ｎ＞２）．萃取回收率８５．７１％，方法回收率９９．７０％～１１０．７％。
A sensitive RP-HPLC method has been developed for the determination of tenoxicam in plasma.After having been extracted by dichloromethane, the tenoxicam plasma sample was separated and determinated on an ODS column with methanol-phosphate buffer-triethylamine(30:69.8:0.2)as mobile phase,and piroxicam as the internal standard.Usign UV detection at 254 nm,the lower limit of detection was 0.1μg/ml,and the calibration curve wes linear between 0.1～30 μg/ml with a regression coefficient of 0.9997.The extraction recovery was 87.51%,while the method recovery was 99.70%～110.7%.The present assay procedure has been applied to monitorirng plasma levels in oral pharmacokinetic studies in. rabbit.The results implieil that tenoxicam was absorbed slowly and also eliminated slowly in the rabbit body.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1995年第3期160-162,共3页
Journal of China Pharmaceutical University
