摘要
用当地分离株与标准毒株研制出传染性支气管炎（ＩＢ）二价油乳苗，免疫雏鸡后第３周，抗体反应呈阳性，第５周可达８ｌｏｇ１０，能分别抵御单一毒株及混合毒株的攻击。ＩＢ二价油乳苗的免疫剂量对雏鸡的免疫反应影响不大，但剂型则有较大影响。双相型油乳苗产生抗体效价较高，持续时间较长，灭活苗抗体产生时间较晚，效价也低于前者．对免疫鸡组织病理学的检查表明．免疫系统各组织在抗原的持续作用下．呈现活跃的应答状态．淋巴细胞数明显多于对照组；胸腺、脾脏、法氏囊等重要免疫器官内都有大量浆细胞及浆母细胞．与血清学检测结果相符。
Abstract Young chicken are vaccinated with IB bivalent oil-emulsion vaccine, which iS composed of the standard strain and the isolate from Tianjin area. IB antibody, which can prevent from the challenge of single strain and mixing strains appears in the third week after vaccination .and can reach 8 log10 till tile fifth week. There is no difference in.antibody production between 0. 2ml and 0. 4ml vaccination dosase but the oil-emulsicn vaccine produces faster and higher antibody titer than inactive vaccine.Histopathology of vaccinated chicken chows that immune system gives active responses to antigen stimulating. There are more iympnocytes in the chicken of experimental group than those of control. Lots of Plasmablastes and plasmacytes can befound in thymus,pspleen,bursa,and so on, Which is in keeping with the results of serological test.
