摘要
应用ＮＤＬａＳｏｔａＮ７９株和ＥＤＳ－７６Ｈ９３１株，分别接种鸡胚和鸭胚增殖病毒．血凝价分别达到≥１：１２８０和≥１：１００００时，收取病毒．经福尔马林灭活．按一定比例加入吐温８０、司本８０、硬脂酸铝和白油，高速搅拌均匀，制成ＮＤ和ＥＤＳ－７６二联疫苗。该苗具有良好的免疫力，其中两种病毒无相互干扰性，接种鸡群无不良反应。疫苗置４～８℃和１５～３０℃分别保存８和６个月不破乳．不分层，颜色无变化。用联苗接种６０００余只父母代种鸡，接种后２５天．ＮＤ和ＥＤＳ－７６抗体可先后达到高峰，接种后９个月，鸡群ＮＤ和ＥＤＳ－７６抗体平均效价仍分别保持在７．０和５．６ｌｏｇ２水平上。对１６～１８周龄产蛋母鸡（皮下注射１ｍｌ／只）应用２０万羽份的联苗证明，疫苗安全．效力确实，使用方便．效益显著。
Abstract ND La Sota N79 strain and EDS-76 H931 strain were inoculated into chicken embryos and duck embryos for virus reproduction. Virus was collected when the hemagglutination valence≥1: 1280and ≥1: 10000 respectively. Collected virus inactivated with Formalin and mixed with certain proportions of Tuwn-80, Span-80, Aluminum Stearate and Leucoil, and then blended to produce NDEDS-76 Oil-adjnvent combined vaccine. Test result showed that the combined combined viccine had a good immunity, no interferences between these two kinds of viruses, no side reaction occured in the inoculated flock. The emulsion does not seperate and had no changes in color after & and 6 months storage under the temperature of 4～8℃ and 15～30℃ respectively. In the 6000 inoculated parents flock, antibodies peak of ND and EDS-76 was achieved successively 25 days after the inoculation, and the average titers of the antibodies maintaned at 7. 0 and 5. 6 log2 9 months after inoculation. lml subcutuneouse injection of the vaccine is enough to 16～18 weeks old commercial flock. 200000 chicken-times application had shown that this vaccine is effective, convenient, safe and reliable, and beneficial.
